吗啡预处理兔心肌MALDI-TOF-MS分析  

Proteomic analysis of morphine rabbit myocardium with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

作　　者：谭嵘[1] 邹定全[1] 常业恬[1] 

机构地区：[1]中南大学湘雅二医院麻醉科,长沙410011

出　　处：《中南大学学报（医学版）》2010年第6期607-611,共5页Journal of Central South University ：Medical Science

摘　　要：目的:对吗啡预处理兔心肌进行基质辅助激光解吸飞行时间质谱(matrix-assisted laser desorption/ionization time-of-flight masss pectrometry,MALDI-TOF-MS)分析。方法:将6只新西兰大白兔随机分为对照组和吗啡预处理组,每组3只。对照组静脉注射生理盐水1mL/kg,吗啡预处理组静脉注射吗啡3mg/kg。用双向凝胶电泳法分离两组24h后的心肌组织蛋白,图像分析后找出差异蛋白。用MAL-DI-TOF-MS鉴定部分差异蛋白。结果:两组的差异明显点共有51个,取15个差异蛋白质进行质谱分析,初步鉴定出8个蛋白质,包括应激蛋白(醛糖还原酶、锌指蛋白312)、信号转导蛋白(Src相关的酪氨酸激酶)、代谢相关蛋白(碳酸酐酶12前体、电子转移黄素蛋白β亚单位、甘油醛-3-磷酸脱氢酶)、炎性反应相关蛋白(肿瘤坏死因子配体超家族成员11)和跨膜转运蛋白。结论:这些差异表达的蛋白可能在吗啡对心肌延迟性保护中发挥作用。Objective To analyze the morphine rabbit myocardium with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry（MALDI-TOF-MS）.Methods Six New Zealand white rabbits were randomly assigned to a control group （Group C） and a morphine group（Group M）.Group C were pretreated with bolus injection of saline 1mL/kg.Group M were pretreated with bolus injection of morphine 3 mg/kg.The myocardium tissue proteins of the rabbits 24 hours after the injection of morphine or saline preconditioned were extracted and separated by two dimensional gel electrophoresis（2-DE）,and the images were analyzed and different proteins were found.Some of the different proteins were determined with MALDI-TOF-MS.Results There were 51 protein spots that displaied quantitive changes in expression（P0.05）,15 protein spots were choosen for MS analysis,and 8 proteins were preliminarily identified.They were aldose reductase,zinc finger protein 312,src related tyrosine kinase,carbonic anhydrase 12 precursor,electron transfer flavoprotein beta-subunit,glyceraldehyde-3-phosphate dehydrogenase,tumor necrosis factor ligand superfamily member 11 and transmembrane emp24 domain-containing protein.Conclusion These proteins may be involved in the cardioprotection of morphine preconditioning.

关 键 词：吗啡 预适应 延迟相 基质辅助激光解吸飞行时间质谱分析 

分 类 号：R541[医药卫生—心血管疾病]