复方别敏中黄芩苷、淫羊藿苷和黄芪甲苷配伍比例的优化  

Optimal combination of baicalin,icariin and Astragalus saponin Ⅰ from a Chinese herbal compound Biminne

作　　者：弓唯一[1] 张新民[1] 沈自尹[1] 胡国让[2] 张素琴[1] 刘闰红[1] 张卫东[3] 董竞成[1] 

机构地区：[1]复旦大学华山医院中西医结合研究所,上海200040 [2]澳大利亚悉尼大学Concord医院免疫与变态反应科 [3]第二军医大学药学院,上海200433

出　　处：《中西医结合学报》2010年第6期541-547,共7页Journal of Chinese Integrative Medicine

基　　金：上海市科学技术委员会攻关项目(No.02DZ19128)

摘　　要：目的:探讨复方"别敏"中黄芩苷、淫羊藿苷与黄芪甲苷3种成分合用的优化配伍比例。方法:采用卵清蛋白腹腔注射建立变应性鼻炎小鼠模型,以脾淋巴细胞增殖反应为指标,利用四唑氮氢氧化物法确定黄芩苷、淫羊藿苷、黄芪甲苷各成分的有效剂量范围;选用U1*0(108)表组方设计所得的3种成分的10种组合进行干预,考察其对脾淋巴细胞增殖的抑制效应,经逐步回归统计分析,获得3种成分合用的最佳配比;通过淋巴细胞增殖反应和对淋巴细胞培养上清液中细胞因子含量检测,验证3种成分的最佳配伍效应是否优于各单一成分。结果:2～10μmol/L黄芩苷、2～10μmol/L淫羊藿苷和1～10μmol/L黄芪甲苷明显抑制致敏小鼠脾淋巴细胞增殖;当黄芩苷、淫羊藿苷、黄芪甲苷以1:2.14:2.65比例配伍时,抑制细胞增殖的效应最明显;进一步的验证研究证实,该配伍对ConA刺激的淋巴细胞增殖的抑制效果及对淋巴细胞上清液中白细胞介素4(interleukin-4,IL-4)、IL-5和γ干扰素(interferon-gamma,IFN-γ)的调节作用明显优于黄芩苷、淫羊藿苷、黄芪甲苷单用的效果(P<0.05,P<0.01)。结论:复方"别敏"中主要有效成分黄芩苷、淫羊藿苷、黄芪甲苷按优化比例合用可最有效地抑制致敏小鼠脾淋巴细胞增殖,调节因变态反应引起的Th1、Th2型细胞因子失衡。Objective： To study the optimal combined ratio of baicalin, icariin and Astragalus saponin Ⅰ from a Chinese herbal compound Biminne. Methods： Firstly, a mouse model of allergic rhinitis was established by intraperitoneal injection of ovalbumin （OVA） and aluminum hydroxide gel suspension, and the effective dose range of baicalin, icariin and Astragalus saponin Ⅰ was detected by 2,3-bis（2-methoxy-4-nitro-5-sulfophenyl）-2H-tetrazolium-5-carboxanilide inner salt method. Secondly, 10 groups of combinations of baicalin, icariin and Astragalus saponin Ⅰ assembled by U＊10（108） form were employed to determine the optimal combination by means of analyzing of the inhibitory effect on the splenocyte proliferation. Finally, the effects of each effective ingredient and the optimal combination were compared by observing the splenocyte proliferation, the contents of interleukin-4 （IL-4）, IL-5, interferon-gamma （IFN-γ） in supernatant of the splenocyte cultures and the ratio of IL-4 to IFN-γ in order to verify the result. Results： Baicalin or icariin at concentrations ranging from 2 to 10 μmol/L, and Astragalus saponin Ⅰ from 1 to 10 μmol/L effectively suppressed the splenocyte proliferation. When the proportion of baicalin, icariin and Astragalus saponin Ⅰ was 1∶2.14∶2.65, the inhibitory effect was most remarkable. Further research confirmed the rationality of the optimal combination. Conclusion： An optimal combination of the major effective ingredients from Chinese herbal compound Biminne most effectively suppresses the proliferation of splenocytes from sensitized mice and regulates the cytokine secreting.

关 键 词：中药复方 变应性鼻炎 细胞增殖 配伍 均匀设计 淋巴细胞 

分 类 号：R289[医药卫生—方剂学]