用电喷雾离子阱质谱法对SFZ-47在家兔体内两主要代谢物的分析  

Identification of Two Major Metabolites of 3 H 1,2 Dihydro 2 (4 methyl phenylamino)methyl 1 pyrrolizinone in Rabbits Using Electrospray Ion Trap Mass Spectrometry

作　　者：钟大放[1,2] 姜浩[1,2] 顾景凯 周慧[1,2] 

机构地区：[1]沈阳药科大学药物代谢与药物动力学实验室 [2]吉林大学分子生物学系

出　　处：《沈阳药科大学学报》1999年第1期1-5,共5页Journal of Shenyang Pharmaceutical University

摘　　要：以ＨＰＬＣ对家兔单剂量口服１００ｍｇ新抗炎镇痛剂ＳＦＺ４７［３Ｈ１，２二氢２（４甲基苯胺基）甲基１吡咯里嗪酮］后尿中的两主要代谢物进行了分离、制备，再用电喷雾离子阱质谱法对代谢物分别进行结构鉴定．结果表明，采用负离子ＥＳＩＭＳ３检测方式，对代谢物ＳＦＺ４７羧基衍生物［４（３Ｈ１。Two major metabolites of a 100 mg oral dose of SFZ 47[3 H 1,2 dihydro 2 (4 methylphenylamino)methyl 1 pyrrolizinone],a new anti inflammatory and analgesic agent,in rabbit urine were separated and prepared with the high performance liquid chromatography,and then the structures of the metabolites were identified by the use of the electrospray ion trap mass spectrometry.It was demonstrated that the structures of the two major metabolites of SFZ 47 in rabbits,the carboxylic derivative of SFZ 47[4 (3 H 1,2 dihydro 1 pyrrolizinone 2 methylamino)benzoic acid]and its acyl β D glucuronide were well characterized with the (-)ESI MS 3 ion trap mass spectrometry.

关 键 词：电喷雾 离子阱质谱 代谢物 SFZ-47 抗炎镇痛剂 

分 类 号：R971.1[医药卫生—药品] R914.1[医药卫生—药学]