间断性阻断肝血流对线粒体功能的影响及机理探讨  

Effect of discontinuous blood interruption on liver mitochondria in rat

作　　者：孙经建[1] 吴孟超[1] 陈汉[1] 钱光相[1] 

机构地区：[1]第二军医大学东方肝胆外科医院

出　　处：《中华普通外科杂志》1999年第1期28-30,共3页Chinese Journal of General Surgery

摘　　要：目的探讨间断性和持续性阻断肝血流对线粒体功能的影响及其作用机理。方法实验大鼠分为持续阻断组、间断阻断组和假处理组，检测其肝线粒体功能、肝组织内钙和脂质过氧化物（Ｌｉｐｉｄｐｅｒｏｘｉｄｅ，ＬＰＯ）含量，以及超氧化物歧化酶（ｓｕｐｅｒｏｘｉｄｅｄｉｓｍｕｔａｓｅ，ＳＯＤ）活性。结果与间断性阻断肝血流相比，持续性缺血再灌注后大鼠肝线粒体呼吸控制比率（ｒｅｓｐｉｒａｔｏｒｙｃｏｎｔｒｏｌｒａｔｉｏ，ＲＣＲ）、磷氧比值（Ｐ／Ｏ）、氧化磷酸化效率（ｏｘｉｄａｔｉｖｅｐｈｏｓｐｈｏｒｙｌａｔｉｏｎｒａｔｅ，ＯＰＲ）和ＳＯＤ活性均显著下降，其值分别为３０４±０１７对２０９±０１５（Ｐ＜００１）、１５１±００９对１２２±０１０（Ｐ＜００５）、２４２±２３对１３４±２３（Ｐ＜００１）及５７３４±３８５Ｕ·ｍｇｐｒ－１对４２２９±３３６Ｕ·ｍｇｐｒ－１（Ｐ＜００５）；而肝组织内钙与ＬＰＯ值均显著升高，分别为３７２±２４μｇ／ｇ对４８５±２９μｇ／ｇ（Ｐ＜００５）及５２０±０１７ｎｍｏｌ·ｍｇｐｒ－１对５８７±０１７ｎｍｏｌ·ｍｇｐｒ－１（Ｐ＜００５）。而在间断性血流阻断组与假处理组之?Objective To evaluate the effect of discontinuous and continuous interruption of blood supply to the liver on mitochondrial function in rat.Methods Rats were divided into three groups:discontinuous interruption,continuous interruption and sham operation.Liver mitochondrial function,contents of calcium and lipid peroxide (LPO),and superoxide dismutase (SOD) activity of the rat were measured.Results Compared with discontinuous blood supply interruption,respiratory control ratio (RCR),P/0 ratio, oxidative phosphorylation rate (OPR) and SOD activity significantly declined and contents of calcium and LPO increased after continuous ischemia reperfusion.They were 3 04±0 17 vs 2 09±0 15 ( P <0 01),1 51±0 09 vs 1 22±0 10 ( P <0 05),242±23 vs 134±23 ( P <0 01),5734±385 U·mgpr -1 vs 4229±336 U·mgpr -1 ( P <0 05),37 2±2 4 μg/g vs 48 5±2 9 μg/g ( P <0 05) and 5 20±0 17 nmol·mgpr -1 vs 5 87±0 17 nmol·mgpr -1 ( P <0 05),respectively.All parameters in discontinuous interruption group have changed insignificantly as compared with sham operative rats.Conclusions The results suggest that discontinuous ischemia reperfusion of liver is less hazardous to mitochondrial function because the calcium equilibrium and activity of SOD remained comparatively normal.

关 键 词：线粒体 过氧化物 肝外科手术 再灌注损伤 

分 类 号：R657.3[医药卫生—外科学]