哈姆林甜橙蔗糖合酶Ⅰ和酸性转化酶基因表达与果实糖积累的关系  

Expression of Sucrose Synthase Ⅰ and Acid Invertase Genes in Relation to Sugar Accumulation in Fruits of Hamlin Sweet Orange

作　　者：王滕旭[1] 李正国[1] 杨迎伍[1] 邓伟[1] 

机构地区：[1]重庆大学生物工程学院基因工程研究中心重庆市高校功能基因与调控新技术重点实验室,重庆400030

出　　处：《热带作物学报》2010年第5期745-749,共5页Chinese Journal of Tropical Crops

基　　金：国家支撑计划项目(2007AA1018)资助

摘　　要：蔗糖合酶(Sucrose synthase,SS)与酸性转化酶(Acid invertase,AI)是甜橙糖代谢过程中的两个关键酶,SS催化尿嘧啶核苷二磷酸葡萄糖和果糖合成蔗糖的可逆反应,AI则不可逆的催化蔗糖分解为果糖和葡萄糖,它们在甜橙糖代谢过程中起着重要作用。笔者以哈姆林甜橙品种为试验材料,采用半定量RT-PCR法,对4个时期哈姆林叶片中的SSI基因和AI基因的表达进行了分析。结果表明,4个时期哈姆林叶片中均可检测到SSI基因和AI基因在转录水平上的表达,但表达量存在差异。SSI基因在果实发育过程中表达量呈"低-高-低-高"趋势。AI基因在哈姆林果实发育成熟过程中的表达逐渐减弱,与果实糖累积呈负相关。Sucrose synthase （SS） and acid invertase （AI） are two key enzymes in sweet orange sugar metabolism.SS catalyzes reversible reaction of uridine diphosphate glucose and fructose into sucrose.AI catalyzes irreversible hydrolysis of sucrose into fructose and glucose.Both enzymes play important roles in orange sugar metabolism.In this study, SSI and AI gene expression in leaves of Hamlin were analyzed in four different developmental stages by semi-quantitative RT-PCR.The results showed that SSI and AI gene expressions were detected in four analyzed stages; however, the levels of their expressions varied in different stages.As fruit development, the expression pattern of SSI in leaves was ＂low-high-low-high＂ while the expression of AI gene was decreased gradually.Therefore, the expression levels of AI in leaves were negatively correlated with the accumulation of sugars in fruit.

关 键 词：哈姆林 蔗糖合酶I基因 酸性转化酶基因 半定量RT-PCR 糖代谢 

分 类 号：Q78[生物学—分子生物学]