香蕉MuMADS2基因表达产物的亚细胞定位  

Subcellular Localization of Expression Products of Banana MuMADS2 Gene

作　　者：刘琳[1,2] 杨晓颖[1,2] 金志强[1] 刘菊华[1] 张浩[1,2] 徐碧玉[1] 

机构地区：[1]中国热带农业科学院热带生物技术研究所农业部热带作物生物技术重点开放实验室,海南海口571101 [2]海南大学农学院,海南海口571101

出　　处：《热带作物学报》2010年第5期777-781,共5页Chinese Journal of Tropical Crops

基　　金：国家自然科学基金项目(30760153;30960232);香蕉公益性行业科研专项(nyhyzx07-029);中央级公益性科研院所基本科研业务费专项基金(ITBBZD0733)资助

摘　　要：对已获得的香蕉MuMADS2基因进行生物信息学和芯片分析表明,该基因编码的蛋白可能作为转录因子定位于细胞核中,是乙烯上游的调控因子,参与调控香蕉果实的成熟过程。为进一步研究该基因功能,构建香蕉MuMADS2基因与绿色荧光蛋白(Green Fluorescent Protein,GFP)基因融合的植物表达载体pCAMBIA1302-MuMADS2,再利用基因枪转化法将其转入洋葱表皮细胞进行亚细胞定位观察。结果表明:该基因表达产物定位于细胞核中,符合转录因子特性。An MADS-box gene named MuMADS2 was isolated from banana fruit with SSH and DNA microarray method and its expression was up-regulated in the early stage of banana fruit after postharvested.Bioinformatics and microarray analysis showed that MuMADS2 gene encoding the protein as a transcription factor might be located in the nucleus and played roles in the upstream of ethylene signaling, involved in the regulation of banana fruit ripening.In order to further study its function,a plant expression vector pCAMBIA1302-MuMADS2 with green fluorescent proteins was constructed and transformed into onion epidermal cells by gene gun method.The result of transient expression showed that MuMADS2 gene was located in the nucleus which was in line with the characteristics of transcription factors.

关 键 词：香蕉 MuMADS2基因 转化 亚细胞定位 

分 类 号：S668.1[农业科学—果树学]