尿中尼古丁含量的高效液相色谱测定法  

Determination of Nicotine in Urine by HPLC

作　　者：冯斌[1] 邵华[1] 单永乐[1] 

机构地区：[1]山东省职业卫生与职业病防治研究院检测与评价中心,250062

出　　处：《职业与健康》2010年第14期1586-1587,共2页Occupation and Health

基　　金：山东省自然科学基金项目(Y2002C30)

摘　　要：目的建立一种实用的高效液相色谱法测定人尿中尼古丁含量的方法。方法在流动相为0.02mol/LH3PO4-KH2PO4(pH值=3)、流速为1.0ml/min、C18反相柱、室温、波长为262nm的条件下,测定尼古丁。结果标准曲线的相关系数>0.9992,加标回收率大于91.5%,变异系数小于6.0%。结论应用该方法测定尿中尼古丁的含量,方法简便、准确、灵敏度高。[Objective]To establish a method for determination of Nicotine in the urine by high performance liquid chromatography（HPLC）.[Methods]Under the condition of mobile phase 0.02 mol/L H3PO4-KH2PO4（pH=3）,velocity of flow 1.0 ml/min,C18 reversed phase column,room temperate,Wavelength 262 nm,the nicotine was determined.[Results]The correlation coefficient of standard curve was more than 0.9992,and the recovery was more than 91.5%,the coefficient of variation was less than 6.0%.[Conclusion]The method is simple,accurate,and sensitive for nicotine determination in urine.

关 键 词：高效液相色谱法 尼古丁 检测 尿液 

分 类 号：R115[医药卫生—公共卫生与预防医学]