高效液相色谱法检测大鼠血浆中盐酸环苯扎林浓度的方法研究  

Determination of cyclobenzaprine hydrochloride in rat plasma by HPLC

作　　者：汪六英[1] 何盛江[2] 刘实[1] 沈小俊[1] 李莉[1] 

机构地区：[1]扬子江药业集团南京海陵药业有限公司,江苏南京210049 [2]中国药科大学,江苏南京210009

出　　处：《现代医药卫生》2010年第13期1924-1925,共2页Journal of Modern Medicine & Health

摘　　要：目的:建立血浆中盐酸环苯扎林含量的测定方法。方法:用高效液相色谱法(HPLC),色谱柱为HypersilBDS-C18(5μm,4.6mm×250mm),乙腈-0.085%磷酸水溶液(三乙胺调pH为6.5)65:35为流动相;流速为1.0mL·min-1;检测波长290nm。结果:盐酸环苯扎林在12.06～241.2ng·mL-1范围为内与峰面积线性关系良好(r=0.9998);对照品溶液48小时内稳定,RSD为1.1%;低、中、高3种血浆浓度的平均回收率为(94.45±5.21)%、(98.64±2.74)%和(99.62±1.36)%。结论:该方法简便,精密度及重现性均较好,分离效果好,该法适应于盐酸环苯扎林血浆样品的快速、大量分析。Objective：To study the determination method of cyclobenzaprine hydrochloride in rat plasma by HPLC.Methods：The separation is performed by Hypersil BDS-C18（5 μm,4.6 mm×250 mm）with the mobile phase of acetonitrile-0.085% phosphoric acid aqueous solution adjusted pH 6.5 by triethylamine （65：35V/V）.While the detection wavelength was 290nm for the determination of cyclobenzaprine hydrochloride.Results：Cyclobenzaprine hydrochloride and other peaks were separated well and the peak shape was presented well.There was the good linearity within the range between 12.06 ng·mL-1~241.2 ng·mL-1 of cyclobenzaprine hydrochloride （r=0.9998）.The reference substance solution presented fair stabilization within 48 h and RSD was 1.1%.The average recovery rates for low,middle and high solutions were （94.45±5.21）%,（98.64±2.74）% and （99.62±1.36）respectively.Conclusion：The method is sensitive,accurate with good reproducibility.And it is suit for the massive and rapid determination of cyclobenzaprine hydrochloride plasma samples.

关 键 词：盐酸环苯扎林 高效液相色谱法 生物样品 

分 类 号：R9[医药卫生—药学]