土壤食细菌线虫对菲降解的影响  

Effects of Bacterial-Feeding Nematode on Phenanthrene Degradation in Soil

作　　者：井永苹[1] 赵莉[1] 陈小云[1] 刘满强[1] 胡锋[1] 李辉信[1] 

机构地区：[1]南京农业大学资源与环境科学学院,南京210095

出　　处：《土壤》2010年第3期372-377,共6页Soils

基　　金：国家环保部和江苏省环保厅项目(江苏省污染土壤调查)资助

摘　　要：以菲作为多环芳烃污染物的代表,通过室内培养试验研究了在菲降解菌恶臭假单胞菌存在与否的条件下,接种食细菌线虫对未灭菌土壤中菲去除的影响。析因设计包括4个处理：单独接种恶臭假单胞菌（B）,单独接种食细菌线虫（N）,同时接种食细菌线虫和恶臭假单胞菌（BN）以及未接种线虫和细菌的对照（CK）;分别在培养后第0、5、14、28天进行破坏性采样,测定土壤中菲的残留量,食细菌线虫和恶臭假单胞菌的数量,土壤FDA（荧光素二乙酸酯）水解酶、过氧化氢酶的活性。结果显示,相比CK,在培养前期（0～14天）,处理N提高土壤中菲的去除率;而在培养后期（14～28天）,BN处理降解菲的优势逐渐显现出来。在培养结束（28天）时,各处理菲的去除率依次为BN（48.2%）〉B（45.1%）〉N（44.4%）〉CK（43.5%）。试验结果还表明,接种食细菌线虫能显著（p〈0.05）促进细菌以及土壤酶活性。总之,食细菌线虫和细菌的交互作用可能促进土壤中菲的降解。A microcosm incubation experiment was conducted to study the effects of bacteria-feeding nematodes, which feed phenanthrene-degrading bacteria （Pseudomonas putida）, on phenanthrene removal from the un-sterilized soil. Phenanthrene was selected as a model of aromatic hydrocarbon （PAH）. The factorial experimental design include four treatments, i.e., soil inoculated with bacteria only （B）, bacteria-feeding nematodes only （N）, both B and N （BN）, and neither B nor N （CK）. Soil was sampled destructively at 0 d, 5 d, 14 d and 28 d after the initiation of incubation. Selected indices include the residual concentrations of phenanthrene, the abundance of bacteria-feeding nematodes and enzyme activities of the catalase, hydrolysis rate of fluorescein diacetate （FDA）. The results demonstrated that bacteria-feeding nematodes inoculated only （N） treatment stimulated phenanthrene removal during the incubation period of 0-14 days, however, the stimulation of BN on phenanthrene removal increased gradually, especially obvious at later incubation period （14-28 days）. At the end of the incubation period, the sequence of phenanthrene removal was： BN （48.15%）〉B （45.06%）〉N （44.43%）〉CK （43.52%）. The results also showed that the inoculation of bacteria-feeding nematodes could significantly （p〈0.5） increase bacteria and soil enzyme activities with the incubation time. In conclusion, the interaction between bacterial-feeding nematodes and bacteria could possibly accelerate phenanthrene degradation in soil.

关 键 词：菲 食细菌线虫 恶臭假单胞菌 土壤酶活性 

分 类 号：S154.38[农业科学—土壤学] X171.5[农业科学—农业基础科学]