高压液相色谱法检测肠道通透性及临床应用  

Detection of Intestinal Permeability by High Performance/pressure Liquid Chromatography

作　　者：何桂珍[1] 舒红[1] 刘卫[1] 周开国[1] 

机构地区：[1]中国医学科学院北京协和医学院北京协和医院肠内肠外营养科,北京100730

出　　处：《现代检验医学杂志》2010年第3期12-14,18,共4页Journal of Modern Laboratory Medicine

基　　金：国家自然基金资助项目（项目编号：30471707）.

摘　　要：目的用高压液相色谱的方法检测肠道通透性和60例健康志愿者和烧伤患者尿中乳果糖和甘露醇的排出率（L／M）。方法选取60例健康志愿者，平均年龄36．5岁（21～60岁）；病人12例烧伤面积大于30％，Ⅲ度面积大于20％的患者，测定伤后1，3，6，12d尿中乳果糖和甘露醇溶液的排出率。健康志愿者和烧伤患者在前1天晚10时起禁食；早8时口服10ml乳果糖和甘露醇溶液（含2．0g乳果糖和1．0g甘露醇），收集口服溶液后6h内的全部尿液。尿样经离心和预处理后，用带脉冲电化学检测器的高压液相离子色谱仪测定，样本分析时间为10min。结果甘露醇和乳果糖标准曲线线性良好，线形的相关系数r^2分别为0．998823和0．999943；方法学的重复性误差（CV％，n=10）：乳果糖（L）3．52，甘露醇（M）1．61；回收率：乳果糖（L）98．4±3％，甘露醇（M）101．1±3（n=10）。60例健康人6h尿中的孔果糖（L）和甘露醇（M）排出率（％）的比值（L／M）是0．036±0．0008。12例烧伤患者第1d的L／M值显著增加（0．221±0．169，P〈0．05），到12d时基本恢复正常（0．036±0．051）。结论带脉冲电化学检测器的高压液相离子色谱仪测定尿中乳果糖和甘露醇的排出率，方法准确、重复性和回收率满意。烧伤患者L／M比值的测定可反映肠黏膜的通透性和肠道屏障功能。Objective Detection of intestinal permeability of 60 healthy volunteers and 12 severe burn patients urine excretion rate of lactulose and mannitol （L/M） by high performance/pressure liquid chromatography （HPLC）. Methods 60 healthy volunteers had the average age of 36.5（21-60） and male and female were 30 respectively. 12 severe burn patients had more than 30% burn area. All subjects had to fast from 10：00pro the day before and took 10 ml of lactulose and manni- tol solution orally （containing 2.0 g lactulose and 1.0 g mannitol） at 8：00 am. All urine of subjects were collected after took solution orally within 6 h. After urine were centrifuged and pretreatmented the samples measured by HPLC with pulsed electrochemical detector. A run analysis time was 10 min. Results Mannitol and lactulose standard curve were good linearity and r^2 were 0. 998 823 and 0. 999 943 respectively. The repeatability error （CV% ,n= 10） of lactulose （L） and mannitol （M） were 3.52 and 1.61% respectively. The rate of recovery were laetulose （L） 98.4±3 and mannitol （M） 101. 1±3（n=10）. The ratio of lactulose and mannltol （L/M） were 0. 036±0. 000 8 in 60 cases of healthy volunteers. The patients L/M ratio value were significant increase （P〈0. 05） on 1 day and returned to normal on 12 day. Conclusion Detection of lactulose and mannitol by the high-performance liquid ion chromatography with pulsed electrochemical detector is accurate ,reproducible and recovery are satisfaction. The ratio of excretion rate in burn patients can reflect the permeability and mucosa barrier function of intestinal.

关 键 词：肠道通透性 乳果糖/甘露醇 高压液相色谱 健康志愿者 

分 类 号：Q503[生物学—生物化学] R446[医药卫生—诊断学]