121例体检者幽门螺杆菌感染免疫表型检测及分型结果分析  

Immunopheno Type Detecting and Typing Results of 121 Individuals with H. pylori Infection in Healthy Examination Crowd

作　　者：陈达伟[1] 戴玉柱[2] 金美彤[2] 江晓肖[1] 张忠夫[3] 孙长贵[2] 成军[2] 

机构地区：[1]解放军第117医院消化内科,杭州310013 [2]解放军第117医院临床实验中心,杭州310013 [3]解放军第117医院健康管理中心,杭州310013

出　　处：《现代检验医学杂志》2010年第3期79-81,共3页Journal of Modern Laboratory Medicine

摘　　要：目的了解体检人群不同年龄幽门螺杆菌（Hp）感染情况、免疫表型和分型的分布特征。方法以年龄进行分组采用^14C-尿素呼气试验（^14C—UBT）、粪便Hp抗原试验对267名体检者进行Hp检测筛选，然后采用免疫印迹试验对体检人群Hp感染者进行免疫表型检测和分型，并对其进行统计学分析。结果267名体检者的总Hp阳性率为45．3％（121／267），且随年龄增高而上升（P〈0．05）；121例Hp感染者中抗CagA-116，KDVacA-95KD，VacA-91KD抗体总阳性率分别为70．2％（85／121），65．3％（79／121）和57．0％（69／121），三种抗体的阳性率在不同年龄组之间差异无统计学意义（P〉0．05）；Ⅰ型和Ⅱ型Hp感染率在体检人群中的百分比分别为71．1%（86／121）和28．9％（35／121），但Ⅰ型和Ⅱ型Hp感染率在体检人群不同年龄组中的构成比存在差异（P〈0．05），低年龄组（〈18岁，18～30岁）和高年龄组（〉60岁）的Ⅱ型Hp感染率高于文献报道的临床病例组。结论体检人群Hp感染率低于慢性活动性胃炎、消化性溃疡等临床病例，且与年龄呈正相关；免疫分型在体检人群中存在特殊的分布特征。Objective To investigate status of H, pylori （Hp） infection, its immunophenotype and subtype distribution in different ages of individuals receiving health examination. Methods 267 individuals receiving healthy examination were divided into six age groups,and then Hp screening tests including^14 C-urea breath test （^14C-UBT） and stool examination for Hp antigens were performed. Subsequently,immunophenotypes and typing of the Hp-positive cases were carried out with Western blotting,and meanwhile statistical analyses were also performed. Results Hip-positive rate of the 267 cases was 45.3% （121/267） ,and the positive rate significantly increased along with the increase of age （P〈0. 05）. The positive rate of anti-CagA-116,anti-KDVacA-95KD and anti-VacA-91KD antibodies in 121 cases of Hp infection was 70. 2% （85/121）, 65.3% （79/121） and 57.0% （69/121）, respectively. Furthermore, there were no significant differences in the positive rates of three antibodies among different age groups （P〉0. 05）. Besides,the infection of type Ⅰ and Ⅱ Hp accounted for 71.1%（86/121） and 28.9% （35/121）,respectively. However,there were significant differences in the positive rates of type Ⅰ and Ⅱ Hp among different age groups （P〈0. 05） ,and the positive rates of type Ⅱ Hp in low age group （〈18 y,18 -30 y） and high age group （〉60 y） was significantly higher than that of clinical cases in references. Conclusion Hp infection rate in individuals receiving healthy examination was lower than that in clinical cases with chronic active gastritis or peptic ulcer ,etc ,and presented a positive correlation with age. Special immune typing distribution was found in people＇s receiving healthy examination.

关 键 词：幽门螺杆菌 ^14C-尿素呼气试验 免疫表型 免疫分型 

分 类 号：R378.99[医药卫生—病原生物学] R392.11[医药卫生—基础医学]