测量的不确定度在临床化学检验中的初步应用  

Initial Application on Measurement Uncertainty of Clinical Biochemistry

作　　者：单斌[1] 王玉明[1] 郭冲[1] 左薇薇[1] 詹淑芬[1] 段勇[1] 

机构地区：[1]昆明医学院第一附属医院检验科,昆明650032

出　　处：《现代检验医学杂志》2010年第3期89-91,94,共4页Journal of Modern Laboratory Medicine

摘　　要：目的初步探索测量的不确定度在临床生化检验中的应用。方法遵循《测量的不确定度表示指南》（GUM），采用A，B类评定方法和两种不同的计算方法，评定实验室主要的常规生化项目（Alb，ALP，ALT，AST，Ca，Crea，Chol，GGT，Glu，Mg，P，UA，TB，TG和TP）的测量不确定度，并对评定结果进行比较。结果以拙内、批阎重复性和方法偏倚为分量所进行的测量不确定度的评估大小主要取决于室间质评的靶值，而以长期重复性和校准品不确定度为分量所进行的评价结果显示两者均构成合成不确定度的主要分量。结论采用A，B类评定方法，考虑仪器测定本身存在的不确定度以及临床检验过程的不确定度2个分量，作为临床检验工作的不确定度研究的探索，为进一步的深入研究提供一点思路和线索。Objective To explore the measurement uncertainty of clinical biochemistry. Methods According GUM, the measurement uncertainty of main routine biochemical items was evaluated by using type A and 13 of evaluation method and two types of calculate methods. Results The uncertainties of routine biochemistry evaluated by within,with-run and bias. It was decided by target of EQA. If used long time CV and uncertainty of calibration to evaluate routine items uncertainty, two could cause all effect the uncertainty. Conclusion For the evaluation of the measurement uncertainty of clinical chemistry by using two types of evaluation method the two uncertainty components are considered ,including the proper uncertainty of machines and uncertainty of measurement process. It can provide some clues for further research.

关 键 词：测量不确定度 临床生化检验 

分 类 号：R446.112[医药卫生—诊断学]