湿疹患者外周血CD4^＋CD25^＋FOXP3^＋调节性T细胞和血清IL-4，IL-10，TGF—β检测及临床研究  

Detection of nTreg Cell and IL-4, IL-10,TGF-β in Peripheral Blood of Eczema Patients and Its Clinical Significance

作　　者：任兴斌[1] 诸葛宝忠[1] 季海生[1] 

机构地区：[1]山东省临沂市人民医院,山东临沂276003

出　　处：《现代检验医学杂志》2010年第3期131-133,136,共4页Journal of Modern Laboratory Medicine

摘　　要：目的通过对湿疹患者外周血单个核细胞（PBMC）中转录调节因子3（FOXP3）阳性的天然调节性T细胞（nTreg，CD4^＋CD25^＋Foxp3^＋）表达率及其血清白介素4（IL-4）、白介素10（IL-10）、转化生长因子β（TGF—β）水平的检测结合心理评定并与对照组进行对比分析，探讨其在湿疹发病过程中的作用及患者发病的可能机制。方法应用流式细胞术（flow cytometry，FCM）和ELISA法对湿疹患者（n=32）、对照组（n=32）分别进行PBMC中nT表达率及血清IL-4，IL-10，TGF-β水平进行检测，同时应用症状自评量表（SCL-90）进行心理评定。结果湿疹患者PBMC中nTreg细胞表达率（％）为4．86±1.69，明显低于对照组6．10±2．21；血清IL-4（96．21±20．07pg／ml），IL-10（12．6±1．95pg／ml）含量高于对照组相应检测值53．93±13．10，9．82±1．34pg／ml；TGF-β 15．76±3．11ng／ml含量明显低于对照组（24．39±4．44ng／ml），各项检测指标两纽之间比较差并均有统计学意义（P〈0．01），患者心理健康状态与对照组比较也有显著性差异。结论nTreg数量降低减弱了对Th2细胞的抑制作用是湿疹患者发病的关键环节，Th1／Th2失衡、细胞因子分泌异常在疾病的发生发展中起重要作用，是湿疹发病和慢性化的重要原因，加强对湿疹患者的心理疏导以减少对患者精神、躯体造成的伤害，提高疗效具有重要临床意义。Objective To detect CD4^＋CD25^＋Foxp3^＋ nTreg cells,IL-4,IL-10 and TGF-β in serum among eczema patients combined with the psychological evaluation and comparative analysis with the control group;to approach the relationship between changes of IL-4 ,IL-10 and TGF-β and onset of eczema;to analyze the role of these changes in onset of eczema and possible mechanism of eczema onset. Methods Detected the number of nTreg cells in PBMC by FCM and detected the level of IL-4,IL-10 and TGF-β in serum by ELISA method while applying symptom self-assessment table （SCL-90） for psychological assessment among eczema patients （n = 32） and control group （n = 32）. Results There was significant decrease of nTreg cells in PBMC among eczema patients （4. 86 %±1.69%） than that among control group （6.10% ± 2.21% ）. The level of IL-4,IL-10 in serum of eczema patients（96. 2±20. 07 pg/ml, 12. 6± 1.95 pg/ml） was significantly higher than that of control group（53.93±13.10 pg/ml,9. 82＋1. 34 pg/ml）and the level of TGF-β in serum of eczema patients（15.76 ±3.11 ng/ml） was significantly lower than that of control group（24.39±4.44 ng/ml）. Mental health of patients was also significantly different with that of control group. Conclusion The decrease of nTreg cells weaken its inhibition to Th2 cells ,which may be important reason of eczema onset. Imbalance of Th1/Th2 and abnormality of cytokine secretion may take important role in onset and chronic development of eczema. Strengthening psychological counseling may reduce the mental,physical harm to patients and improve efficacy.

关 键 词：湿疹 天然调节性T细胞(nTreg CD4^+CD25^+Foxp3^+) 白介素4(IL-4) 白介素10(IL-10) 转化生长因子β(TGF—β) 流式细胞术(FCM) 

分 类 号：R758.23[医药卫生—皮肤病学与性病学] R392.11[医药卫生—临床医学]