脂多糖诱导肥大细胞释放HMGB1研究  

Extracellular release of HMGB1 in lipopolysaccharide-induced mast cells and related signaling pathways

作　　者：杨岚[1] 李蕾[2] 邵俊良[2] 许飞[1] 陈国千[2] 

机构地区：[1]南京医科大学附属无锡妇幼保健院检验科,江苏无锡214002 [2]南京医科大学附属无锡人民医院医学检验科,江苏无锡214023

出　　处：《南京医科大学学报（自然科学版）》2010年第7期919-922,共4页Journal of Nanjing Medical University(Natural Sciences)

基　　金：江苏省自然科学基金资助(BK2006027)

摘　　要：目的:研究脂多糖(LPS)对肥大细胞高迁移率族蛋白B1(HMGB1)释放的诱导作用及其胞内信号通路。方法:采用小鼠肥大细胞株P815,观察不同剂量LPS诱导后细胞培养上清液中HMGB1含量和细胞HMGB1mRNA表达水平的变化,及不同剂量丝裂原活化蛋白激酶(MAPK)信号通路抑制剂(SB203580、SB202190、U0126和PD98059)对LPS诱导后HMGB1胞外释放的影响。HMGB1含量采用酶联免疫吸附试验检测。结果:LPS诱导后细胞培养上清液中HMGB1含量明显升高,并与LPS剂量、诱导时间有关;100μg/LLPS分别诱导8、24、48h后,HMGB1mRNA表达水平明显增强(P<0.01);SB203580和SB202190对LPS诱导HMGB1释放有明显的抑制作用(P<0.05),但U0126和PD98059不显示抑制作用。结论:LPS诱导肥大细胞释放HMGB1,其释放机制与胞内p38MAPK信号通路有关。Objective：To study the extracellular release of high mobility group box 1（HMGB1）in mast cells induced by lipopolysaccharide（LPS）and related intracellular signaling pathways.Methods：The changes of HMGB1 concentration in the culture medium and HMGB1 mRNA expression of P815 mast cells were investigated using enzyme-linked immunosorbent assay（ELISA）and RT-PCR method after stimulation with various concentrations of LPS.The effects of MAPKs pathway inhibitors（SB203580,SB202190,U0126 and PD98059）on extracellular release of HMGB1 were observed in LPS-induced mast cells.Results：LPS induced extracellular release of HMGB1 in mast cells in a dose-and time-dependent manner.The level of HMGB1 mRNA expression significantly was increased by stimulation with 100 μg/L LPS for 8,24 or 48 hours（P0.01）.SB203580 and SB202190 partly inhibited HMGB1 release in LPS-induced mast cells（P0.05）.But no effects were observed in U0126 and PD98059 treated group.Conclusion：LPS can induce mast cells to release HMGB1,in which the intracellular p38 MAPK signaling pathway is involved.

关 键 词：高迁移率族蛋白B1 肥大细胞 内毒素 丝裂原活化蛋白激酶 

分 类 号：R392.12[医药卫生—免疫学]