Lamin B1在邻苯二甲酸二丁酯致大鼠尿道下裂发生中的表达变化  

Differential expression of Lamin B1 in the hapospadiac male rats induced by di-n-butyl phthalate

作　　者：魏云飞[1] 常俊锴[1] 冯宁翰[1] 张炜[1] 

机构地区：[1]南京医科大学第一附属医院泌尿外科,江苏南京210029

出　　处：《南京医科大学学报（自然科学版）》2010年第7期928-931,共4页Journal of Nanjing Medical University(Natural Sciences)

基　　金：国家自然科学基金资助(30872596)

摘　　要：目的:利用邻苯二甲酸二丁酯(di-n-butyl phthalate,DBP)胚胎晚期暴露导致SD胎鼠尿道下裂发生的动物模型,验证Lamin B1在尿道下裂与正常胎鼠阴茎中的差异表达,以进一步探讨尿道下裂的发生机制。方法:妊娠14～18天SD雌鼠20只,随机分为2组,实验组和对照组分别给予DBP800mg/(kg·d)和大豆油2ml/d灌胃,孕期第19天剖宫产后记录雄性胎鼠肛门和生殖器之间的距离(AGD)、出生体重(BW)及尿道下裂发生数,取雄性胎鼠阴茎,运用免疫印迹法和免疫组织化学方法分析Lamin B1的表达情况。结果:实验组AGD/BW与对照组相比有显著性差异(P<0.05);尿道下裂发生率为40%;Lamin B1在对照组的相对表达量为0.923±0.013(n=10),实验组为0.593±0.014(n=10),差异有统计学意义(P<0.05);Lamin B1主要定位于尿道上皮细胞,对照组染色强度明显强于实验组。结论:DBP灌胃期间改变了尿道组织中Lamin B1的表达,影响了细胞迁移和尿生殖褶的融合,这可能是尿道下裂发生的原因之一。Objective：To verify the differential expression of Lamin B1 in the penis of rat offspring after maternal exposure to di-nbutyl phthalate（DBP）in order to explore the mechanism of hypospadias.Methods：Twenty pregnant SD rats were randomly divided into two groups acting as experiment group and control group,which were respectively given DBP and soybean oil gavage at the dose of 800 mg/（kg·d）and 2 ml/d since the 14～18 th day of pregnancy,anogenital distance（AGD）,birth weight（BW）,hypospadias incidence were recorded,and penises of fetal rats were harvested at GD19.The expression of Lamin B1 was analyzed by Western blot and im munohistochemistry.Results：The incidence of hypospadias was 40%,and the AGD/BW of experiment group was significantly lower than control group（P 0.05）,and the expression of Lamin B1 had statistical difference between two groups（P0.05）.Lamin B1 was mainly located in urethral epithelium in the developmental phase of the penis,and the control group staining intensity was stronger than the experimental group.Conclusion：Maternal exposure to DBP can reduce the Lamin B1 expression in urethral epithelium,affect cellular migration and urethral fold fusion,and then result in the occurrence of hypospadias.

关 键 词：LAMIN B1 邻苯二甲酸二丁酯 尿道下裂 阴茎 

分 类 号：R321.6[医药卫生—人体解剖和组织胚胎学] Q513[医药卫生—基础医学]