锌胁迫对不知火杂柑和椪柑叶片光合特性及超微结构的影响  

Effects of Zinc Stress on Leaf Photosynthetic Characteristic and Ultrastructure of Shiranuhi and Ponkan

作　　者：肖家欣[1,2] 徐春丽[1] 齐笑笑[1] 张绍铃[2] 

机构地区：[1]安徽师范大学生命科学学院生物环境与生态安全安徽省高校省级重点实验室,安徽芜湖241000 [2]南京农业大学园艺学院,江苏南京210095

出　　处：《激光生物学报》2010年第3期297-302,共6页Acta Laser Biology Sinica

基　　金：中国博士后基金项日(20080430172);安徽省自然科学基金项日(070411004)

摘　　要：采用砂基培养法,运用CI-340便携式光合测定仪、H-7650透射电子显微镜研究了不同锌浓度如0 mg/L(锌缺乏)、0.05 mg/L(对照)和0.5 mg/L(锌过量)处理下不知火杂柑(简称不知火)和椪柑叶片叶绿体色素含量、净光合速率及超微结构的变化。结果表明:(1)锌缺乏处理的不知火及锌过量处理的椪柑叶片叶绿素(a+b)和类胡萝卜素含量、锌缺乏与锌过量处理的两者叶片净光合速率均相对低于对照。(2)锌缺乏处理下,两者叶片叶绿体出现变形或空室化,其中椪柑叶绿体内淀粉粒和质体小球增多,细胞核及线粒体正常,而不知火淀粉粒少,细胞核中出现许多黑色小颗粒物质。锌过量处理下,两者叶片叶绿体出现空室化或叶绿体膜模糊,线粒体解体,其中椪柑叶绿体内质体小球增多,淀粉粒少;而不知火淀粉粒明显增多、增大,基粒片层膨胀、松弛。可见,锌胁迫对两者叶片叶绿体色素、净光合速率及超微结构有着明显的影响,其影响程度因品种而异。Changes of leaf chloroplast pigments contents, net photosynthetic rate and ultrastructure of shiranuhi （Citrus reticulata×（C. reticulata×C. sinenesis）） and ponkan （Citrus reticulata Blanco） under sand culture were researched under Zn2＋ of 0 mg/L（zinc deficiency）, 0.05 mg/L （control） and 0.5 mg/L（Zn excess） treatments using the CI-340 portable photosynthesis system and H-7650 transmission electron microscope. The results showed that leaf chlorophyll （a＋b） and carotenoid contents of shiranuhi’under zinc deficiency, ponkan under zinc excess, and Leaf net photosynthetic rates of both under zinc deficiency and excess treatments were relatively lower than those of controls. Under zinc deficiency treatment, distortion or vacuolization was observed in leaf chloroplast of both, as to ponkan, starch grains and platoglobuli increased, nucleus and mitochondria were normal, whereas starch grains were rare in chloroplast of shiranuhi, and many small black deposits were found in nucleus. Under zinc excess treatment, vacuolization or blurred chloroplast membrane was detected in chloroplast of both, mitochondria disintegrated, in stroma of chloroplast of ponkan, platoglobuli increased, and few starch grains were found, while starch grains obviously increased and swelled, and swollen thylakoid lamella were found in chloroplast of shiranuhi. It’s suggested that there exist obvious effects of zinc stress on leaf chloroplast pigments contents, net photosynthetic rate and ultrastructure of both, the influence degrees were different for different cultivars.

关 键 词：锌胁迫 不知火杂柑[Citrus reticulata×(C. reticulata×C. sinenesis)] 椪柑(Citrus RETICULATA Blanco) 光合特性 超微结构 

分 类 号：S666[农业科学—果树学] Q945[农业科学—园艺学]