高效液相色谱法测定罗红霉素颗粒剂的含量  

Determination of roxithromycin granules by RP HPLC

作　　者：钟志东[1] 历士旺[1] 

机构地区：[1]天津市儿童医院

出　　处：《中国医院药学杂志》1999年第1期30-31,共2页Chinese Journal of Hospital Pharmacy

摘　　要：目的：探讨反相高效液相色谱法测定罗红霉素颗粒剂含量的方法。方法：在Ｃ１８柱上以乙腈０．０８３ｍｏｌ·Ｌ－１醋酸铵甲醇（５５∶２３∶２８），并以醋酸调节ｐＨ６．８为流动相，在２１５ｎｍ波长处检测。结果：在０．５～７ｍｇ·ｍｌ－１浓度范围内，浓度与样品峰面积呈良好线性关系（ｒ＝０．９９９５），样品回收率为９９．９２％（ｎ＝６，ＲＳＤ０．８９％）。结论：方法简便可靠。OBJECTIVE:To probe into an RP HPLC for determination of roxithromycin granules.METHODS:The Lichrosorb RPC 18 analytical column was used.An aliquot of aqueous buffer phase was chromatographed using acetonitrite 0.083 mol·L -1 ammonium acetate solution methanol(55∶23∶28) as mobile phase.The wavelength used for detection was 215 nm.RESULTS:The detection ranges were 0.5 ～7 mg·ml -1 .The average recovery of roxithromycin was 99.92% ( n=6,RSD = 0.89% ).CONCLUSIONS:The method are simple and convenient.

关 键 词：罗红霉素 反相 高效液相色谱法 颗粒剂 

分 类 号：R978.15[医药卫生—药品] R927.2[医药卫生—药学]