猪前脂肪细胞诱导分化过程中PPARγ、DECR1和ECHS1基因表达模式  

Expression Models of PPARγ,DECR1 and ECHS1 Genes during Porcine Preadipocyte Differentiation

作　　者：刘海峰[1] 阎振鑫[1] 董涵[1] 李学伟[1] 

机构地区：[1]四川农业大学动物科技学院,四川雅安625014

出　　处：《四川农业大学学报》2010年第2期215-218,共4页Journal of Sichuan Agricultural University

基　　金：农业部现代农业产业技术体系建设专项项目(NYCYTX-009);四川省科技攻关项目(2006-YZGG-15)

摘　　要：采用胶原酶消化法分离猪皮下前脂肪细胞,以含50 nmol/L胰岛素、100 nmol/L地塞米松、0.25 mmol/L 3-异丁基-1-甲基黄嘌呤、100 nmol/L罗格列酮的分化培养液对前脂肪细胞进行诱导分化,借助实时定量RT-PCR方法检测了PPARγ、DECR1和ECHS1 3基因的表达模式,结果发现:PPARγ、DECR1和ECHS1 3基因均于诱导分化后48 h达到表达高峰,此时的表达量分别是诱导前的93.0,5.4和7.8倍;PPARγ同DECR1和ECHS1两基因表达量变化趋势间的相关系数分别为0.92和0.97,DECR1同ECHS1的相关系数为0.94,均达到极显著相关(P<0.01)。实验结果表明:前脂肪细胞在分化过程中细胞内脂肪酸生物合成和脂肪酸β-氧化同时发生,以维持细胞的稳态。We isolated preadipocyte from subcutaneous adipose tissue of two days old piglets by using collagenase digestion approach.The preadipocyte were cultured in different mediums supplemented with 50 nmol/L insulin,100 nmol/L dexamethasone,0.25 mmol/L 1-methyl-3-isobutylxanthine and 100 nmol/L rosiglitazone.Total RNA were extracted from the cells and the mRNA expression level of PPARγ,DECR1 and ECHS1 genes were measured by using real-time quantitative RT-PCR method.The results revealed that the expression levels of PPARγ,DECR1 and ECHS1 were up-regulated to the maximum at 48 h,and the change folds of these genes were 93.0,5.4,3.3 and 7.8,respectively.Strikingly,the good positive correlations among the mRNA expression levels of PPARγ,DECR1 and ECHS1 across time points were observed,coefficient correlation were 0.92,0.97,0.94,respectively.We present tentatively conclusions that,in order to maintain cell homeostasis,the fatty acid biosynthesis and fatty acid β-oxidation were raised simultaneously during differentiation of preadipocytes.

关 键 词：前脂肪细胞 定量PCR 脂肪酸生物合成 脂肪酸β-氧化 猪 

分 类 号：S828[农业科学—畜牧学] Q344.13[农业科学—畜牧兽医]