福安水牛线粒体DNA D-loop区遗传多样性分析  

Genetic Diversity on mtDNA D-loop Region of Fu'an Buffalo

作　　者：张瑞光 梁建平[2] 陈云 林斌 陈诚 苗永旺[2,3] 

机构地区：[1]福建省福安市畜牧兽医站,福建福安355000 [2]云南农业大学动物科学技术学院,云南昆明650201 [3]云南大学云南省生物资源保护与利用重点实验室,云南昆明650091

出　　处：《云南农业大学学报（自然科学版）》2010年第4期525-529,共5页Journal of Yunnan Agricultural University:Natural Science

基　　金：云南省应用基础研究重点项目(2007C0003Z);云南省应用基础研究计划面上项目(2006C0034M);国家自然科学基金项目(30660024);国家高技术研究发展计划(863计划)项目(2008AA101001)

摘　　要：采用PCR产物直接测序的方法对20头福安水牛的线粒体DNA(mtDNA)D-LOOP区全序列进行了测定,并对所得数据进行了比对和分析。结果共检测到16种单倍型,48个核苷酸多态位点,其中单一变异位点9个,简约信息位点39个。在所分析的序列中,T,C,A和G的平均含量分别为27.9%,25.6%,31.9%和14.6%,核苷酸多样度为0.019 46±0.002 88,单倍型多样度为0.957±0.032,序列间平均核苷酸差异数是17.238,结果显示福安水牛线粒体DNA遗传多样性丰富。系统发育和遗传距离分析显示:福安水牛与河流型水牛存在较大差异,其自身聚为支持率极高的两大支,即世系A和世系B,揭示福安水牛存在两个母系血统来源。In this study,the D-loop region sequences of mitochondrial DNA（mtDNA） of 20 Fu＇an buffalo samples were determined by direct sequencing of PCR product.The sequences were aligned and analyzed to investigate the genetic variation and genetic divergence within Fu＇an buffalo population.Sixteen mitochondrial haplotypes and 48 polymorphic sites included 9 singleton polymorphic sites and 39 parsimony informative sites were detected.The average percentage values of base composition of T,C,A and G were 27.9%,25.6%,31.9% and 14.6%,respectively.The nucleotide diversity,haplotype diversity and the average number of nucleotide differences within the population were 0.019 46±0.002 88,0.957±0.032 and 17.238,respectively.The results indicated there were rich variations in Fu＇an buffalo population.Genetic distance and phylogenetic analysis showed that there was remarkable genetic divergence between Fu＇an buffalo and river buffalo,and Fu＇an buffalo composed of two lineages,viz.lineage A and lineage B.This indicated that there might be two maternal origins for Fu＇an buffalo.

关 键 词：福安水牛 MTDNA D-LOOP 遗传多态性 遗传分化 

分 类 号：S823.83[农业科学—畜牧学]