多粘菌素B预包被ELISA反应板检测抗内毒素抗体  

INCREASE OF THE SENSITIVITY OF ELISA FOR ANTI LPS ANTIBODIES BY PRECOATING THE MICROTITER PLATES WITH POLYMYXIN B

作　　者：许伟群[1] 朱苹[1] 苏东辉[1] 黄爱明[1] 刘梁英[1] 

机构地区：[1]福建医科大学微生物学教研室

出　　处：《中国人兽共患病杂志》1999年第1期47-49,共3页Chinese Journal of Zoonoses

摘　　要：由于细菌脂多糖（ＬＰＳ）水溶性差，用传统的ＥＬＩＳＡ检测抗－ＬＰＳ抗体抗原用量大，敏感性不理想。我们用能和ＬＰＳ结合的多粘菌素Ｂ（ＰＭＢ）预先包被微量反应板，可使随后加入的ＬＰＳ（光滑型和粗糙型）包被效率增加，检测抗－ＬＰＳ抗体（单克隆抗体和动物免疫血清）的敏感性显著增加（最高达１２８倍）。Conventional ELISA for anti lipopolysaccharide(LPS)antibodies is not ideally sensitive because of the poor solubility of bacterial LPS.A new ELISA by precoating the microtiter plates with ploymyxin B was developed,a cyclic antibiotic that is able to bind with LPS,to improve the coating efficiency of LPS.In comparison with the conventional ELISA /in which LPS was directly coated onto the plates,the new method detected much higher(128-fold as the highest)anti LPS titers in murine antisera and monoclonal antibody raised against Re LPS.

关 键 词：抗体 ELISA 多粘菌素 内毒素 细菌脂多糖 PMB 

分 类 号：R631.404[医药卫生—外科学] R446.62[医药卫生—临床医学]