滇川钉螺的同工酶谱比较  

COMPARATIVE STUDY ON THE ISOENZYME PATTERNS OF ONCOMELANIA SNAILS IN SICHUAN AND YUNNAN PROVINCES

作　　者：王少海[1,2] 何立[1,2] 康在彬 

机构地区：[1]湖北药检高等专科学校寄生虫学教研室 [2]湖北医科大学寄生虫学教研室

出　　处：《中国人兽共患病杂志》1999年第1期52-54,共3页Chinese Journal of Zoonoses

基　　金：国家自然科学基金

摘　　要：目的研究滇川工地钉螺的同工酶谱特征及其亲缘关系，为钉螺分类提供资料。方法对采自四川省天全、绵竹、彭县；云南省大理、楚雄、洱源的钉螺分别进行解剖、匀浆，应用聚丙烯酰胺凝胶对匀浆物电泳，分别进行苹果酸脱氢酶（ＭＤＨ）和酯酶同工酶（ＥＳＴ）染色。结果ＭＤＨ共分离出３条酶带，６地钉螺酶谱特征完全一致，Ｒｆ值为０．１６４，０．２０８，０．３０９，ＥＳＴ共分离出１１～１４条酶带，６地钉螺完全一致的酶带有８条，Ｒｆ值为０．０７４，０．２９６，０．３７３，０．４０８，０．４９７，０．５２０，０．６０１，０．６５１。结论２省间曾被命名为不同种群的钉螺亲缘关系密切；同时因长期的地理阻隔。Aim To study isoenzyme patterns and relationship of Oncomelania snials between Sichuan and Yunnan Provinces.Methods The snails were collected from Tianquan,Mianzhu,Pengxian counties of Sichuan Province,Dali,Chuxiong,Eryuan counties of Yunnan Province.All these snails were electrophoresed by means of slab polyacrylamide gel,then were stained on malate dehydrogenase(MDH)isoenzyme and esterase(EST).Results 3 enzyme bands were separated on MDH from snails of 6 counties and their enzyme patterns were entirely identical,Rf values were 0 164,0 208,0 309.11～14 enzyme bands were entirely identical,Rf values were 0 074,0 0296,0 373,0 408,0 497,0 520,0 601,0 651.Conclusion The snails population from the 2 provinces was very closely related,but still some differences existed in the characteristics of encyme patterns between snails of different regions because of long period geographic isolation.

关 键 词：钉螺 苹果酸脱氢酶 酯酶 同工酶 分类 

分 类 号：R184.38[医药卫生—流行病学] Q959.212[医药卫生—公共卫生与预防医学]