大白菜显性核复等位基因雄性不育恢复基因Ms^f的SSR标记  

SSR Mapping of the Ms^f,a Multiple-allele Male-fertility Restorer Gene in Chinese Cabbage(Brassica campestris L.ssp.pekinensis)

作　　者：王丽丽[1] 魏鹏[1] 刘志勇[1] 李承彧[1] 王玉刚[1] 冀瑞琴[1] 冯辉[1] 

机构地区：[1]沈阳农业大学园艺学院,沈阳110866

出　　处：《园艺学报》2010年第6期923-930,共8页Acta Horticulturae Sinica

基　　金：国家自然科学基金项目(30671414);国家‘863’计划项目(2006AA10Z170)

摘　　要：为筛选大白菜核复等位基因遗传的雄性不育恢复基因Msf的SSR标记,用基因型为MsfMs的雄性不育两用系‘AB01’的可育株自交,得到恢复基因型纯合个体MsfMsf。再用其与基因型为msms的自交系‘a20’杂交和连续回交,获得BC4分离群体。筛选了104对SSR引物,获得3个与雄性不育恢复基因Msf连锁的SSR标记syau_m13、syau_m14和BRMS-040。经群体验证和连锁分析,Msf基因位于R07连锁群上,3个标记位于Msf基因的同一侧,距Msf基因的遗传距离分别为6.7、6.7和8.6cM。In order to construct a molecular-assisted selection（MAS）system for breeding the multiple-allele male-sterile lines in Chinese cabbage,a BC4 mapping populations was constructed with the fertile plant（MsfMsf） which was selfing progenies of the genetic multiple-allele male-sterile AB as the female parent and inbred line ＇a20＇（msms）which is the male parent.Screening 104 SSR primer pairs was performed with near isogenic lines（NILs）.The results showed that three SSR markers named syau_m13,syau_m14 and BRMS-040 were found to be linked to the target Msf.Based on the genetic linkage analysis,the Msf gene was located on the linkege group R07.Finally,a genetic map of the Msf gene was constructed covering a total interval of 8.6 cM.The three markers were located on the same side of the gene at distances of 6.7,6.7 and 8.6 cM,respectively.

关 键 词：大白菜 核复等位基因雄性不育 恢复基因 SSR 

分 类 号：S634.1[农业科学—蔬菜学]