红景天多糖对猪精子冷冻保护效果的研究  

Protective Effect of Rhodiola Polysaccharides on Freezing Boar Spermatozoa

作　　者：张树山[1,2] 李青旺[3] 胡建宏[1] 江中良[1] 戴建军[1,2] 吴彩凤[1,2] 张廷宇[1,2] 张德福[1,2] 

机构地区：[1]上海农业科学院畜牧兽医研究所,上海201106 [2]上海农业遗传育种重点实验室动物遗传工程研究室,上海201106 [3]西北农林科技大学动物科技学院,杨凌712100

出　　处：《农业生物技术学报》2010年第3期519-525,共7页Journal of Agricultural Biotechnology

基　　金：上海市农业遗传育种重点实验室开方课题(Shagb2008-03);上海市农科院青年科技发展基金(2009(15));上海市科技兴农推广项目(2007-3-7);转基因生物新品种培育重大专项(2009ZX08006-014B;2008ZX08006-005);上海市科委科技成果转化项目(103919N1800)共同资助

摘　　要：研究旨在探索红景天多糖以及三种添加方案对猪精子冷冻保护效果的影响。实验1中,在冷冻保存Ⅰ液中分别添加红景天多糖分别为0、2mg/L、4mg/L、6mg/L、8mg/L和10mg/L;实验2中,在冷冻保存Ⅱ液中添加红景天多糖,浓度同实验1;实验3基于实验1和2,对比了三种红景天添加方案对猪精子冷冻效果的影响。研究结果表明:1、红景天多糖较适宜添加浓度为6mg/L,能明显提高猪冷冻精子品质,并能为猪精子提供较好的抗氧化作用;2、三种添加方案中,在Ⅰ液中添加(方案A)对猪冷冻精子保护效果明显优于方案B和C(P<0.05)。In this study, the effects of different concentrations and three adding proposals of rhodiola polysaccharides on freezing boar spermatozoa were tested in three experiments. Experiment 1, rhodiola polysaccharides were added into freezing extenderⅠ, and the concentrations were 0, 2 mg/L, 4 mg/L, 6 mg/L, 8 mg/L and 10 mg/L respectively; Experiment 2, rhodiola polysaccharides were only added into freezing extender Ⅱ, and the concentrations were same as experiment 1; Experiment 3, based on the above, three adding proposals of rhodiola polysaccharides on freezing boar spermatozoa were tested. Results： 1. the optimum concentration of rhodiola polysaccharides was 6 mg/L, and could improve the post-thawing quality and antioxidation for boar spermatozoa during the freezing-thawing process; 2. among three adding proposals, proposal A was superior to proposal B and C （P0.05）.

关 键 词：猪精液 冷冻保存 红景天多糖 精子品质 

分 类 号：S823.34[农业科学—畜牧学]