^(99m)Tc直接法标记抗心肌肌钙蛋白I单克隆抗体  

Direct labeling of AcTnIMA with ^(99m)Tc

作　　者：李建华[1] 李殿富[1] 张寄南[2] 匡琴芳 

机构地区：[1]南京医科大学第一附属医院核医学科,210029 [2]南京医科大学第一附属医院心脏科,210029 [3]南京森科医药技术有限公司

出　　处：《江苏医药》2010年第12期1426-1429,共4页Jiangsu Medical Journal

摘　　要：目的探索99mTc直接法标记抗心肌肌钙蛋白I单克隆抗体(AcTnIMA)的方法 ,并研究标记产物的体外稳定性。方法用氯化亚锡(SnCl2)还原法进行AcTnIMA标记,并用正交实验设计筛选抗体量、SnCl2量、反应体系pH值及放射性活度四个因素的最佳组合;用纸层析法测定标记产物的标记率;用柱层析法测定标记物中99mTc-AcTnIMA的纯度;将标记物在室温(22℃)下放置0、2、4、6h,观察其体外稳定性。结果 99mTc直接法标记AcTnIMA的最佳标记条件为抗体量30μg,SnCl2量80μg,反应体系pH值7.0,放射性活度50mCi,标记率可达99%以上;标记物中99mTc-AcTnIMA的纯度较高;标记产物放置6h后,标记率仍大于95%。结论用99mTc直接法标记AcTnIMA简单高效,标记产物在体外有很好的稳定性。Objective To explore the methodology of direct labeling of anticardiac troponin I monoclonal antibody（AcTnIMA） with ^99mTc and investigate the stability of ^99mTc-AcTnIMA in vitro. Methods AcTnIMA,reduced by SnCl2,was labeled with ^99mTc.The best synthesic condition of the 4 factors-quantity of AcTnIMA and SnCl2,pH and radioactivity,was screened by orthogonal experimental design.The labeling efficiency of the product was measured by paper chromatography,and the purity of ^99mTc-AcTnIMA was determined by column chromatography.Then the stability of the product was observed at room temperature（22℃） at 0,2,4,6 h.Results The labeling efficiency was more than 99%,and its best experimental condition was described as AcTnIMA 30 g,SnCl2 80 g,pH 7.0 and radioactivity 50 mCi.Purity of ^99mTc-AcTnIMA in the product was fairly high.The labeling efficiency was still greater than 95% at 6 h. Conclusion ^99mTc direct labeling of AcTnIMA is simple and efficient,and the product is of high stability in vitro.

关 键 词：^99MTC 标记 抗心肌肌钙蛋白I单克隆抗体 

分 类 号：R817[医药卫生—影像医学与核医学]