菠萝蛋白酶预处理对大鼠心肌缺血-再灌注损伤的保护作用  

Protective effect of bromelain on myocardial ischemia-reperfusion injury in rats

作　　者：林伟[1] 彭亚飞[1] 孙辉[1] 钟玲[1] 

机构地区：[1]福建医科大学附属协和医院心脏病科,福州350001

出　　处：《江苏医药》2010年第12期1430-1433,共4页Jiangsu Medical Journal

摘　　要：目的探讨菠萝蛋白酶(Br)对大鼠心肌缺血-再灌注(I-R)损伤的保护作用。方法 32只雄性SD随机均分4组:A组假手术对照;其他3组开胸穿线阻断左前降支(LAD)冠状动脉30min后再灌注2h建立心肌I-R损伤模型。B组缺血前连续15d腹腔注射磷酸盐缓冲液(PBS)预处理;C组改用含Br的PBS腹腔注射预处理;D组仅结扎LAD30min,I-R2h。连续心电图监测ST段改变,再灌注2h末抽血检测血清肌酸激酶同工酶(CK-MB)与乳酸脱氢酶(LDH),用伊文思蓝及2,3,5-氯化三苯基四氮唑(TTC)双重染色方法测定心肌梗死(MI)及缺血面积,TUNEL法测心肌细胞凋亡指数(AI),Westernblot法检测磷酸化蛋白激酶B(P-AKT)表达。结果 C组CK-MB、LDH、MI面积和心肌细胞AI明显小于B、D组,而P-AKT的表达高于B、D组(P<0.01)。结论 Br预处理可通过增加P-AKT的磷酸化而对I-R心肌起保护作用。Objective To investigate the protective effects of bromelain （Br） on myocardial ischemia-reperfusion（I-R） injury.Methods Thirty-two SD rats were divided into 4 groupps with 8 rats each.The myocardial I-R rat model was established by occlusion of left arterial decending for 30 min and reperfusion for 2 hours in groups of B,D and C.Group A was sham operated.PBS preconditioning with PBS via intraperitoneal injection for 15 consecutive days before ischemia in group B,Br plus PBS was used in group C instead of PBS alone as in group B.Group D was treated with I-R only.The deviations of ST-segment on ECG were recorded during study.Serum creatine kinase brain isozyme（CK-MB） and lactate dehydrogenase（LDH） were measured at 2 hours during reperfusion.Myocardial infaction（MI） and risk area were determined by Evans blue and TTC staining.Myocardial cell apoptotic index（AI） was determined by TUNEL.The change of phospho-protein kinase B（P-AKT） was determined by Western blot.Results Serum CK-MB and LDH,MI size and AI were less,but P-AKT was higher,in group C than those in groups of B and D（P〈0.01）.Conclusion Br preconditioning may protect the myocardium from I-R injury by increasing P-AKT.

关 键 词：菠萝蛋白酶 心肌缺血-再灌注损伤 磷酸化蛋白激酶B(P-AKT) 

分 类 号：R54[医药卫生—心血管疾病]