金黄色葡萄球菌α溶血素诱导脐静脉内皮细胞凋亡机制的研究  被引量:4

Apoptosis mechanism of human umbilical vein endothelial cells induced by Staphylococcus aureus α-hemolysin

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作  者:刘婷婷[1,2] 刘从森[1,2] 管俊昌[1,2] 闵宏林[1,2] 李凤云[1,2] 夏佩莹[1,2] 

机构地区:[1]蚌埠医学院病原生物学教研室 [2]安徽省感染与免疫重点实验室,蚌埠233000

出  处:《中国人兽共患病学报》2010年第6期569-571,共3页Chinese Journal of Zoonoses

基  金:安徽省教育厅自然科学研究计划项目(No.2006KJ393B);安徽省自然科学基金联合项目(No.01043706)

摘  要:目的了解金黄色葡萄球菌(金葡菌)α溶血素诱导脐静脉内皮细胞凋亡机制。方法将不同浓度金葡菌α溶血素感染脐静脉内皮细胞8h后,检测TNF-α、caspase-3及caspase-8的产生量,同时检测加入TNF-α抗体、caspase-3和caspase-8抑制剂后的细胞凋亡率。结果金葡菌α溶血素感染脐静脉内皮细胞8h后,TNF-α、caspase-3、caspase-8的产生量与对照组相比差异有统计学意义(P<0.01);而加入TNF-α抗体、caspase-3和caspase-8抑制剂后凋亡率明显降低(P<0.01)。结论金葡菌α溶血素诱导脐静脉内皮细胞凋亡的机制可能是通过TNF-α介导的caspase-8及caspase-3激活的外源性死亡因子受体途径,这为我们进一步研究金葡菌L型垂直感染与治疗提供了新的思路。To study the apoptosis mechanism of human umbilical vein endothelial cells(HUVEC)induced by Staphylococcus aureus(S.aureus)α-hemolysin,HUVEC were infected by S.aureus α-hemolysin in different concentration for 8 hours.The quantity of TNF-α,caspase 3 and caspase 8 in supernatant were measured by colorimetric assay.After adding antibody against TNF-α and the inhibitor of caspase 3 or caspase 8,the apoptosis rates were detected by flow cytometry at the same time.The quantity of TNF-α,caspase 3 and caspase 8 were increased significantly in HUVEC infected with α-hemolysin compared to the control(P0.01).However,the apoptosis rate of HUVEC was significantly inhibited by antibody against TNF-α and inhibitor of caspase 3 or caspase 8(P0.01).It's suggested that the apoptosis mechanism of HUVEC induced by S.aureus α-hemolysin is probably by means of the exogenous death receptor activated by TNF-αmediated caspase 3 and caspase 8,which provide a new approach for further studies on vertical infection and treatment of S.aureus L-forms.

关 键 词:金黄色葡萄球菌 α溶血素 脐静脉内皮细胞 凋亡 机制 

分 类 号:R378.1[医药卫生—病原生物学]

 

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