血管紧张素Ⅱ对心肌梗塞大鼠成纤维细胞的核酸、胶原合成影响的研究  

Effect of angiotensin Ⅱ on the syntheses of DNA, RNA and collagen protein in isolated fibroblasts from myocardium-infarcted rats

作　　者：申景平[1] 雷立权 高广道 李瑞峰[1] 李炯 张庆殷[2] 

机构地区：[1]山东医科大学病理生理教研室 [2]山东医科大学附属医院 [3]西安医科大学病理生理教研室

出　　处：《中国病理生理杂志》1999年第1期5-7,共3页Chinese Journal of Pathophysiology

基　　金：国家"八五"攻关和国家自然科学基金

摘　　要：目的和方法：本实验利用酶法分离、培养成年大鼠心肌成纤维细胞（Ｆｂｓ），观察了血管紧张素Ⅱ（ＡｎｇⅡ）在不同处理因素下对Ｆｂｓ的３Ｈ－ＴｄＲ、１４Ｃ－ＵＲ、３Ｈ－Ｐｒｏ掺入率的影响。结果：在相同浓度（１０７ｍｏｌ／Ｌ）ＡｎｇⅡ作用下，ＭＩ组Ｆｂｓ对上述３种标记底物的掺入率均显著高于假手术（ＳＯ）组（Ｐ＜０．０５）。ＡｎｇⅡ的上述作用，可被特异性ＡｎｇⅡ拮抗剂［１８］ＡｎｇⅡ和血管紧张素抗肽（Ａｎｇ－ＡＰ）完全阻断，却不能被Ｌｏｓａｒｔａｎ完全阻断。结论：ＡｎｇⅡ可直接作用于心肌Ｆｂｓ，促进Ｆｂｓ的ＤＮＡ、ＲＮＡ和胶原蛋白的合成。ＭＩ组大鼠Ｆｂｓ对ＡｎｇⅡ反应性显著高于ＳＯ组，介导ＡｎｇⅡ对Ｆｂｓ的作用除ＡＴ１外。AIM and METHOD: Isotope-labelled substrate incorporation method was used to investigate the effect of angiotensin Ⅱ(Ang Ⅱ) on the syntheses of DNA, RNA and collagen protein of isolated fibroblasts(Fbs) from myocardial infarction (MI) and sham operated (SO) rats in different conditions. RESULTS: The incorporating rates of 3H-thymidine, 14 C-uridine and 3H-proline of Fbs from MI group were significantly higher than that of SO group, respectively. These effects of Ang Ⅱ on Fbs were abolished completely either by angiotensin antagonists, 1 8 Ang Ⅱ, or by angiotensin antipeptide. On the other hand,angiotensin receptor antagonist, losartan, can not thoroughly eliminate the above-mentioned effects of Ang Ⅱ. CONCLUSIONS: (1) Ang Ⅱ promoted the syntheses of DNA, RNA and collagen protein in Fbs; (2) The responsiveness of Fbs to Ang Ⅱ from MI rats was higher than that of normal rats; (3)AT 1 receptor is not the only substype which regulates the action of Ang Ⅱ to Fbs metabolism.

关 键 词：心肌梗塞 血管紧张素II 成纤维细胞 大鼠 

分 类 号：R542.220.2[医药卫生—心血管疾病]