氧自由基在肝缺血再灌注损伤中的作用及川芎嗪的影响  

Role of oxygen free radicals in reperfusion injury following hepatic ischemia and effect of ligustrazin

作　　者：王万铁[1] 林丽娜[1] 徐正[1] 李东[1] 王宗敏[1] 

机构地区：[1]温州医学院病理生理教研室

出　　处：《中国病理生理杂志》1999年第1期39-41,共3页Chinese Journal of Pathophysiology

基　　金：浙江省教委科研基金

摘　　要：目的：探讨氧自由基在肝缺血再灌注损伤（ＨＩＲＩ）中的作用及川芎嗪的防护作用。方法：应用ＨＩＲＩ家兔及肝癌手术患者，观察超氧化物歧化酶（ＳＯＤ）、谷胱甘肽过氧化物酶（ＧＳＨ－ＰＸ），黄嘌呤氧化酶（ＸＯ）、谷丙转氨酶（ＡＬＴ）活性、丙二醛（ＭＤＡ）含量、肝细胞形态学变化及川芎嗪对上述指标的影响。结果：ＨＩＲＩ时ＳＯＤ、ＧＳＨ－ＰＸ活性显著下降（Ｐ＜０．０５和Ｐ＜０．０１），ＸＯ、ＡＬＴ活性及ＭＤＡ含量明显升高（Ｐ＜０．０５和Ｐ＜０．０１），肝细胞形态学发生异常变化；使用川芎嗪后，上述指标的异常变化均明显减轻，其差异有显著和非常显著（Ｐ＜０．０５和Ｐ＜０．０１）。结论：氧自由基是ＨＩＲＩ的重要发病学因素，而川芎嗪通过清除氧自由基可减轻ＨＩＲＩ。AIM:To explore the role of oxygen free radicals (OFR) in hepatic ischemia-reperfusion injury (HIRI) of rabbits and humans and the protective effect of ligustrazin (LGT). METHODS: Changes of several parameters and effects of LGT on it were observed during HIRI in 20 rabbits and 18 patients who suffered from cancer of the liver and were scheduled for elective hepatic surgery, parameters of which included superoxide dismutase (SOD) glutathione peroxidase (GSH-PX) xanthine oxidase (XO) alanine aminotransferase (ALT) and malondialdehyde (MDA) as well as morphological changes of hepatocytes.RESULTS: SOD and GSH-PX activities decreased significantly ( P <0.05) and very significantly ( P <0.01), XO and ALT activities as well as MDA concentration increased remarkably ( P <0.05 and P <0.01), and abnormal morphological changes of hepatocytes were obvious during HIRI; with treatment of LGT, the abnormal changes of all above parameters were diminished remarkably ( P <0.05 and P <0.01). CONCLUSION: It is indicated that OFR may be one of the primary causes for HIRI, LGT can attenuate HIRI by eliminating OFR.

关 键 词：氧自由基 再灌注损伤 川芎嗪 肝缺血 

分 类 号：R657.302[医药卫生—外科学] R364.12[医药卫生—临床医学]