大叶榉顶芽诱导与增殖培养  

Phyllogen induction and cluster bud multiplication of Zelkova schneideriana Hand.-Mazz.

作　　者：汪灵丹[1] 张日清[1] 金晓玲[1] 

机构地区：[1]中南林业科技大学林学院,湖南长沙410004

出　　处：《中南林业科技大学学报》2010年第6期75-79,共5页Journal of Central South University of Forestry & Technology

基　　金：中南林业科技大学青年科学基金项目"大叶榉组织培养与工厂化育苗技术研究"(2008003B)

摘　　要：为解决大叶榉组织培养中萌芽难、增殖系数低的难题,选取健康、饱满的顶芽为外植体,配合不同激素配比对顶芽进行诱导萌芽、增殖培养的研究。结果表明:不同培养基和激素浓度对顶芽萌芽和增殖培养均影响不同,其中最佳顶芽萌芽培养基是WPM+BA1.0 mg/L,萌芽率最高可达98.3%;最佳增殖培养基是WPM+4.0BA mg/L,最大增值系数为15。By selcting healthy and plump tip buds of Zelkova schneideriana Hand.-Mazz.as explant,and matching different concentrations of hormone,the germination induction and multiplication culture of tip buds were studied.The experimental result indicates： the best media for shoot inducement was derived as WPM added with 1.0 mg/L of BA and the highest germination rate reached 98.3%;The bud cluster was multiplicated rapidly on the WPM medium with 4.0 mg/L of BA and the mean multiplication factor reached 15.

关 键 词：林学 林木育种 大叶榉 组织培养 诱导萌芽 增殖培养 组织培养 

分 类 号：S792.19[农业科学—林木遗传育种]