微囊化细胞冻存的研究进展  

Cryopreservation of microencapsulated cells

作　　者：余松林[1] 韩宝三[1] 杜志勇[1] 吴旭波[1] 吴薇[1] 王加祥[1] 黄芳[1] 李宏为[1] 沈柏用[1] 彭承宏[1] 

机构地区：[1]上海交通大学医学院附属瑞金医院消化外科研究所,上海交通大学医学院附属瑞金医院普通外科器官移植中心,上海市200025

出　　处：《中国组织工程研究与临床康复》2010年第25期4713-4716,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：国家“十一五”高新技术研究发展计划--“863”资助项目(2008AA02Z417);国家自然科学基金重点资助项目(20434030);国家自然科学基金资助项目(30772105,20074031);上海市科委自然科学基金资助项目(07ZR14076)~~

摘　　要：背景:细胞微囊化为细胞大规模、高活性体外培养及长期存储提供了新的途径,低温保存是目前保存细胞的重要方法,技术日新月异,复苏细胞在临床和基础研究中的应用越来越多。目的:分析近年来微囊化细胞冻存的相关研究,对微囊化细胞冻存技术的发展作一总结。方法:以"微囊,冻存"为检索词,检索中国期刊网(1979/2010)及维普数据库(1989/2010),限定文章语言种类为中文;以"Cryopreservation,Microencapsule"为检索词,检索PubMed数据库(1979/2010),限定文章语言种类为English。纳入含有微囊化细胞冻存的研究,排除其他形式细胞冻存的研究,结果以各种细胞微囊化后进行冻存处理后产生作用为指标,共检索到43篇文献。结果与结论:随着生物人工肝与以及其他细胞移植研究的深入,对微囊化细胞的需要量将显著增加,微囊化细胞的低温保存技术已成为保正其顺利应用的关键技术之一。目前,微囊化细胞的低温保存技术已经开展了不少研究,有多项研究结果表明复苏后微囊化细胞的存活率和生物学功能都能保持较好的水平,但相关机制仍未完全阐清,各种冻存方法还需要优化。BACKGROUND：Cell microencapsulation provides a new approach for a large scale,highly active in vitro culture and long-term preservation method of cells.Cryopreservation of microencapsulated cells is an important preservation method at present.Resuscitated cells become more and more applicable in clinical and basic researches.OBJECTIVE：To analyze researches related to cryopreservation of microencapsulated cells in recent years,and to summarize the development of technology on cryopreservation of microencapsulated cells.METHODS：Using ＂Microcapsules,Cryopreservation＂ in Chinese as the key words,CNKI（1979/2010） and VIP database（1989/2010） were retrieved.Using ＂Cryopreservation,Microencapsules＂ in English as the key words,PUBMED database（1979/2010） was retrieved.The retrieval languages are limited to Chinese and English respectively.Articles about cryopreservation of microencapsulated cells were included,excluding other forms of cryopreservation research.The index is the effect after the cryopreservation of a variety of microencapsulated cells.Totally 43 papers were screened out.RESULTS AND CONCLUSION：With the development of the bioartificial liver and other cell transplantation research,the requirements of the microencapsulated cells will significantly increase.The cryopreservation technology of microencapsulated cells has become one of key technologies on guaranteeing its smooth application.Currently,there are a lot of researches regarding the cryopreservation technology of microencapsulated cells,many studies show that the cell viability and biological function can maintain a good level after resuscitation of microencapsulated cells,but related mechanisms are not yet fully clarified,so as to have a need to optimize the various cryopreservation methods.

关 键 词：微囊化细胞 低温保存 冻存保护剂 降温 复苏 综述文献 

分 类 号：R318[医药卫生—生物医学工程]