豚鼠左心室流出道神经分布的形态学观察  

Morphmetric Observation of the Distribution of Nerve Fibre in Left Ventricular Outflow Tract in Guinea-pig

作　　者：胡兴光[1] 李建东[1] 吴靖芳[1] 葛赋贵[1] 

机构地区：[1]河北北方学院基础医学院,河北张家口075000

出　　处：《河北北方学院学报（医学版）》2010年第3期1-4,共4页Journal of Hebei North University:Medical Edition

摘　　要：目的:观察左心室流出道慢反应自律细胞分布区域神经纤维分布特点,探讨其自律性活动是否接受自主神经的调控。方法:将豚鼠左心室流出道可引导出自律性慢反应电位的部位制成标本,采用免疫组织化学染色,光镜观察该部位5-羟色胺(5-HT)、神经肽Y(NPY)能神经纤维的分布。结果:在主动脉瓣膜根部和心室肌交界的自律细胞团周围含有丰富5HT、NPY能阳性神经纤维,呈点状膨体和细线状纤维,在细胞团内也可偶见5-HT、NPY阳性神经纤维。结论:左心室流出道的自律细胞团周围及细胞团内有阳性5HT、NPY能神经纤维分布,结合NE对该部自律性慢反应电位有明显影响,表明左心室流出道慢反应自律细胞的活动可能受交感神经纤维的调控。Objective：To observe the distribution of nerve fibre in left ventricular outflow tract（LVOT）and the region whether to accept the regulation of autonomic nerve fibers.Methods：We made the part of left ventricular outflow tract in which autorhythmicity slow response action potentials could be lead into a specimen,and stained by immunohistochemical staining.We observed 5-HT and NPY-containing nerve fibers under light microscope.Results：Lunar cells clump was observed in the border area between aortic valve root and ventricular muscle by light microscope.We could see a large number of 5-HT and NPY-positive nerve fibers by immunohistochemistry staining and the shape of which is linear or fascicular around cell mass,occasionally they appeared in the cell mass.Conclusion：5-HT and NPY-positive nerve fibers by immunohistochemistry staining existed in LVOT,which indicated these cell clusters in the left ventricular outflow tract might accept the regulation of sympathetic nerve.

关 键 词：豚鼠 左心室流出道 神经肽Y 5-羟色胺 神经组织 

分 类 号：R329.3[医药卫生—人体解剖和组织胚胎学]