基于转录本组和蛋白质组数据的日本血吸虫候选疫苗分子序列的预测和分析  

Prediction and analysis for the vaccine candidates against Schistosoma japonicum based on the sequence data of schistosoma transcriptome and proteome

作　　者：贾侃[1] 赵犇鹏[1] 张艳丽[2] 邵东华[2] 冯新港[2] 林矫矫[2] 李利珍[1] 

机构地区：[1]上海师范大学生命与环境科学学院,上海200234 [2]中国农业科学院上海兽医研究所,上海200241

出　　处：《上海师范大学学报（自然科学版）》2010年第3期299-308,共10页Journal of Shanghai Normal University(Natural Sciences)

基　　金：国家重点基础研究发展计划973课题(2007CB513108)

摘　　要：预测了可能模拟致弱疫苗免疫保护性效果的日本血吸虫抗原分子,为实验鉴定抗日本血吸虫候选疫苗分子提供基础.实验基于已发表的辐照尾蚴转化的童虫的特征性转录本组(曼氏血吸虫)和蛋白质组(日本血吸虫)序列,用BLASTP算法等搜寻来源于正常日本血吸虫童虫和成虫的转录本组和蛋白质组中的序列;应用在线软件、相关数据库及文献对这些序列进行序列注释分析.结果获得了与辐照尾蚴转化的童虫特征性表达的分子同源或一致的日本血吸虫序列131条,它们较高比例地出现在正常童虫高表达的分子集类中;进一步文献分析预测得到了52个日本血吸虫候选疫苗分子,它们的序列特征与存在于血吸虫体被的一些分子和已知的抗血吸虫疫苗分子的相类似.用数据挖掘技术获得了一批日本血吸虫候选疫苗分子,为进一步实验研究奠定了基础.Objective To predict Schistosoma japonicum vaccine candidate antigen molecules which possibly simulate the protective effect induced by radiation-attenuated vaccine against schistosome using data mining technology, and provide basis for further experimental validation of the predicted antigens. Methods Candidated sequences were selected by BLASTP searching normal Schistosoma japonicum schistosomula＇ s or adult worms＇ transcriptome and proteome sequences being homologous with the sequences from characteristic transcriptome （ Schistosorna mansoni ） and proteome （ Schistosoma japonicum） of sehistosomula transformed from irradiated cercaria; then annotation and analysis of candidated sequences were carried out using online softwares, related databases and scientific documents. Results 131 sequences of Schistosoma japonicum were obtained, which were homologous or consistent with molecular sequences of schistosomula transformed from irradiated cercaria. The analysis of scientific documents indicated that, among the 131 predicated sequences, 52 molecules could be designated as Schistosoma japonicum vaccine candidate antigens, which are of similar sequence features to that of some antigen molecules located in Schistosome tegument. Conclusion A set of Schistosoma japonicum vaccine candidate molecules were predicated using data mining technology, that laid a sound basis for the further experimental validation of the predicted molecules.

关 键 词：日本血吸虫 转录本组 蛋白质组 候选疫苗分子 预测 

分 类 号：Q811.4[生物学—生物工程]