小承气汤中肉桂酸和大黄酸的含量测定  

Determination of the Contents of Cinnamic Acid and Rhein in Xiaochengqi Decoction by HPLC

作　　者：李康[1] 陈润莉[2] 贲永光[1] 戴京晶[2] 王媛媛[3] 

机构地区：[1]广东药学院.药科学院,广东广州510006 [2]广东省深圳市福田区疾病预防控制中心,518003 [3]广东药学院.临床医学院,广东广州510006

出　　处：《时珍国医国药》2010年第6期1314-1315,共2页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金青年科学基金(No.30801515);广东药学院人才引进基金和重点培养对象基金资助(No.2007YKX10)

摘　　要：目的建立小承气汤中肉桂酸和大黄酸的HPLC含量测定方法。方法采用Apollo C18柱（150 mm×4.6 mm,5μm）,流动相为乙腈和0.011%冰乙酸溶液,梯度洗脱,时间20 min,流速1 ml/min,检测波长为254 nm。结果肉桂酸和大黄酸的线性范围分别为0.07－0.40μg和0.48－1.92μg,精密度为0.22%和0.19%,平均回收率分别为97.3%和96.9%。结论建立的方法简便、灵敏、准确,可用于小承气汤的质量控制。Objective To establish an RP-HPLC method for determination of cinnamic acid and rhein in Xiaochengqi Decoction.Methods Apollo C18 column（150 mm×4.6 mm,5 μm）was used with the mobile phase of acetonitrile and 0.011% acetic acid aqueous solution in gradient mode.The flow rate was 1.0ml/min,and the detection wavelength was set at 254nm.Results The calibration curve was linear in the range of 0.07~0.40 μg（r=0.999 2） for the cinnamic acid and 0.48~1.92 μg for the rhein（r=0.999 2）.The inner-day RSD was 0.22% and 0.19%,and the average recovery was 97.3% and 96.9%,respectively.Conclusion This method is accurate,simple and sensitive,and can be used for the quality control of Xiaochengqi Decoction.

关 键 词：高效液相色谱 小承气汤 肉桂酸 大黄酸 

分 类 号：R284.2[医药卫生—中药学]