壳聚糖对参芪水煎液成分纯化的影响  

Influence of Chitosan Purification on the Components of Shenqi Aqueous Extract

作　　者：张来华[1] 朱盛山[1] 袁旭江[1] 王博[1] 张志鹏[1] 刘艳[1] 

机构地区：[1]广东药学院中药开发研究所,广东广州510006

出　　处：《时珍国医国药》2010年第6期1316-1318,共3页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(No.30672670);粤港的招标项目(No.20070111-2)

摘　　要：目的研究壳聚糖对参芪水煎液成分纯化的影响。方法以总多糖含量、固含物的量为指标,采用正交设计法进行研究,并结合HPLC指纹图谱法进行分析。结果壳聚糖用量对参芪水煎液中多糖具有显著影响,由正交法可知最佳纯化条件为A3B1C2D3,该工艺总多糖含量为49.26%,固含物的量为55.58%。HPLC指纹分析可知不同工艺纯化液的指纹图谱全貌具有良好的相似性,只在5,6,9,10,12号色谱峰的含量上存在一定差异。结论壳聚糖对参芪水煎液化学成分具有良好的纯化作用。Objective To study the Chitosan purification influence on Shenqi aqueous extract＇s components.Methods By means of determining polysaccharide and solids contents,orthogonal experiment and HPLC method were used to study and analyze.Results The content of Chitosan had significant influence in polysaccharide,and the best purification process was A3B1C2D3,whose polysaccharide content and solids content achieved 49.26% and 55.58% respectively;the nine different technology fingerprints showed that those fingerprints had favourable similarity,and only had some diffrence on the number of 5、6、9、10、12 chromatographic peaks.Conclusion Chitosan can play an excellent role in purification of Shenqi aqueous extract＇s components.

关 键 词：参芪 壳聚糖 纯化 多糖 指纹图谱 

分 类 号：R284.1[医药卫生—中药学]