中草药干品和鲜品基因组总DNA提取及质量比较研究  

Total Genomic DNA Extraction from Dried and Fresh Herbs and Quality Comparison

作　　者：姚庆收[1] 刘秀珍[1] 武玉永[1] 蒋继来[1] 

机构地区：[1]滨州医学院.药学院,山东烟台264003

出　　处：《时珍国医国药》2010年第6期1570-1572,共3页Lishizhen Medicine and Materia Medica Research

摘　　要：目的探讨中草药干品和鲜品总DNA的提取方法,并比较干、鲜品总DNA质量之间的差别,为后续研究奠定基础。方法分别采用SDS法和改良的CTAB法提取6种中草药干品和鲜品总DNA,并对其进行核酸含量测定、电泳检测和酶切反应检测。结果改良CTAB法提取的DNA质量要高于SDS法,在干品和鲜品样品间,干品提取的DNA质量要高于鲜品。利用CTAB法提取鲜品和干品的各6种DNA样品,进行酶切反应得到了清晰的酶切条带。结论改良的CTAB法能够获得高质量的中草药总DNA,且该方法得到的干品DNA比鲜品DNA质量更高,酶切验证二者都可以用于进一步的实验分析。Objective To explore the best total genomic DNA extraction method from the dried and fresh herbs,and to compare the DNA quality as a foundation for future research.Methods SDS and modified CTAB methods were used to get DNA,and the content of nucleic acid,and agarose gel electrophoresis and enzyme digestion were used.Results DNA was higher quality using modified CTAB method to extract,and could get higher quality of total genomic DNA from dry samples.It showed a clear enzyme digestion bands using six kinds of DNA by modified CTAB method extracted from fresh and dried samples of herbs.Conclusion It can be get high-quality of total genomic DNA by the modified CTAB method,and can be used for further experiment analysis.

关 键 词：DNA提取 SDS法 CTAB法 液氮 

分 类 号：R284.2[医药卫生—中药学] R2-03[医药卫生—中医学]