PCR法在早期梅毒诊断中的应用  

Application of PCR in Diagnosis of Primary Syphilis

作　　者：文伟[1] 

机构地区：[1]长沙血液中心,湖南长沙410001

出　　处：《实用预防医学》2010年第7期1400-1401,共2页Practical Preventive Medicine

摘　　要：目的提高早期梅毒诊断的敏感性和特异性。方法采用PCR技术对梅毒螺旋体(Tp)的DNA多聚酶Ⅰ基因(polA)特异性片段进行扩增,共检测385份生殖器溃疡分泌物,同时做暗视野镜检和TpELISA血清学试验。结果 PCR法共检测出阳性75例,敏感性和特异性分别为74.5%、99.3%;PCR法与暗视野镜检及TpELISA结果比较差异有统计学意义。结论 PCR法在早期梅毒诊断中具有快速、有效、结果可靠的优点,可作为血清学的补充试验。Objective To improve the sensitivity and specificity in diagnosis of primary syphilis. Methods Treponema pallidum POR was performed to amplify the specific fragment of DNA polymerase I gene （polA）, 385 samples of genital ulcer secretions were detected by POR; meanwhile, the dark - field microscopy and Tp ELISA were applied to detect the secretions and serum specimens from the same patients respectively. Results Of the 385 tests performed, positive POR resuits were obtained for 75 patients. The sensitivity and specificity of POR were,74.5% and 99.3%, respectively. There were statistically significant differences between POR and dark - field microscopy, Tp ELISA. Conclusions The T. pallidum PCR test is fast, efficient, and reliable. It is a useful addition to serology for the diagnosis of primary syphilis.

关 键 词：梅毒螺旋体 PCR 早期梅毒 DNA多聚酶Ⅰ基因 

分 类 号：R759.1[医药卫生—皮肤病学与性病学]