邻苯三酚自氧化法测定SOD活性中检测波长的优化  

Wavelength Optimization in Deter Minating the Activity of SOD Based on the Reaction of Pyrogallol Autoxidation

作　　者：万军[1] 黄国钧[2] 周霞[1] 

机构地区：[1]西南交通大学生命科学与工程学院,四川成都610031 [2]成都中医药大学,四川成都610072

出　　处：《安徽农业科学》2010年第14期7315-7315,7381,共2页Journal of Anhui Agricultural Sciences

基　　金：中央高校基本科研业务费专项资金资助(SWJTU092T29)

摘　　要：[目的]确定邻苯三酚自氧化法测定SOD活性的波长。[方法]采用双光束紫外可见分光光度计分别对不同时间点及时间段邻苯三酚自氧化中间产物吸光度进行测定。[结果]邻苯三酚自氧化初始中间产物的吸收度在320nm处线性好、灵敏度高。[结论]320nm可作为邻苯三酚自氧化法测定SOD活性的测定波长。[Obiective]The aim was to ascertain the best wavelength in reaction of pyrogallol autoxidation. [Method]The absorbance of intermediate was determined at different time points and time period by ultraviolet spectrophometry. [Result]The absorbance of intermediate at 320 nm was good linear response and high sensitivity. [Conclusion]The best wavelength in reaction of pyrogallol autoxidation was 320 nm.

关 键 词：邻苯三酚自氧化法 SOD 活性 波长 

分 类 号：R917[医药卫生—药物分析学]