番茄果实成熟相关基因SlPEL和SlAPL的表达特性  

Isolation and Expression of Two Fruit Ripening Related Genes SlPEL and SlAPL in Tomato(Solanum lycopersicum)

作　　者：肖东[1,2] 肖阳[3] 蔡应繁[4] 邓小峰[1,5] 吴锁伟[1] 郑旭[1,3] 李晚忱[5] 吴翠平[1,2] 费章君 牛应泽[2] 杨建平[1,4] 

机构地区：[1]中国农业科学院作物科学研究所,北京100081 [2]四川农业大学农学院,四川雅安625014 [3]中国农业科学院研究生院,北京100081 [4]重庆邮电大学生物信息学院,重庆400065 [5]四川农业大学玉米研究所,四川雅安625014 [6]Boyce Thompson Institute for Plant Research,Cornell University

出　　处：《作物学报》2010年第7期1135-1143,共9页Acta Agronomica Sinica

基　　金：国家转基因生物新品种培育重大专项(2008ZX08010-002);国家高技术研究发展计划(863计划)项目(2008AA10Z121);重庆市自然科学基金(CSTC2009BA1088);中央级公益性科研院所基本科研业务费专项(2060302-2);教育部留学回国人员启动费;农业部回国人员择优资助项目资助

摘　　要：分析27个代表番茄不同发育阶段和生物反应的组织特异性及含有152 635个独立EST数据库的数码表达,发现果胶裂解酶基因(pectate lyase,SlPEL)和番茄AP2 Like(SlAPL)的转录受果实成熟的调节。以授粉后不同发育时期的番茄(品种为美味樱桃)果实为试材,用半定量PCR和荧光实时定量PCR分析SlPEL的表达模式,结果表明,授粉后12d,其表达水平明显上升;授粉后16～18d,达到第一个小高峰;28d到最高峰;从28d到完全成熟逐步下降到第一个小高峰的水平。SlAPL的表达模式与SlPEL类似,但其表达启动的时期迟于SlPEL。从授粉后25d,SlAPL转录启动;授粉后28～32d,其转录水平上升到第一个小高峰;39d达到最高峰,以后到完全成熟略有下降。该研究也印证利用EST的数据库进行基因数码表达分析的可行性。During ripening, fruits undergo complicated developmental changes with varying physiological and biochemical activities that affect color, flavor, and texture. In tomato, these changes include accumulation of ethylene, the red pigment lycopene and the cell wall hydrolase, polygalacturonase （PG）. Recently, genes of PG, pectin esterase （PE）, ACC synthetase, ACC oxidase, and ethylene syshetase etc. from tomato, banana, Carica papaya, peach, and mango have been cloned. In this study, a large tomato expressed sequence tag （EST） dataset （152 635 in total） from 27 different EST libraries was analyzed to gain insights into digital gene expressions of two fruit ripening related genes SIPEL and SIAPL in tomato. Their expression profiles were detected using semi-quantitative RT-PCR assays and real-time RT-PCR assays in tomato （cv. Meiweiyingtao） during fruit development and ripening. SIPEL expression increased in the fruit at 12 days after pollination, and reached the small peak 4-6 days later and the larger peak at 28 days after pollination; then dropped to the level of the small peak until red ripening stage. As SIPEL, SIAPL expression was also induced by the fruit ripening. But SIAPL expressed two weeks later than SIPEL. SIPEL expression achieved the small peak at 28-32 days after pollination and the large peak at 39 days after pollination; and kept the higher levels until red ripening stage. The results confirmed that the digital gene expression using different EST libraries is an effective way to check the research value genes.

关 键 词：番茄 果胶裂解酶基因 番茄APETALA2 Like基因 表达分析 

分 类 号：S641.2[农业科学—蔬菜学]