氧化型低密度脂蛋白对骨髓源性平滑肌样细胞小凹蛋白1表达的抑制作用及其与清道夫受体A的关系  

Mechanisms of foam cell formation in smooth muscle cell derived from bone marrow stem cells

作　　者：严鹏科[1] 李世煌 段才闻[3] 谭湘萍[1] 梅峥嵘[1] 司徒冰[1] 肖国宏[1] 

机构地区：[1]广州医学院第三附属医院,广东广州510150 [2]郴州市第四人民医院心内科,湖南郴州423000 [3]南华大学药物药理研究所,湖南衡阳421001

出　　处：《南方医科大学学报》2010年第6期1250-1252,共3页Journal of Southern Medical University

基　　金：湖南省科技计划项目(200606);广东省中医药科研项目(200829);广东省医学科研基金(200884)

摘　　要：目的建立骨髓间质干细胞(BMSC)分化的平滑肌细胞模型,探讨平滑肌源性泡沫细胞小凹蛋白1表达及其与清道夫受体A的关系。方法采用贴壁法从SD大鼠骨髓中分离BMSC,并诱导BMSC分化,80 mg/L ox-LDL处理BMSC分化的平滑肌细胞72 h,蛋白免疫印记法检测细胞清道夫受体SR-A、胆固醇逆转运蛋白ABCA1和小凹蛋白Caveolin-1的表达变化。结果 BMSC-SMC能大量蓄积胆固醇酯,形成泡沫细胞。经80 mg/L ox-LDL处理72 h后,BMSC-SMC中SR-A表达明显增加,ABCA1和caveolin-1显著减少。结论在ox-LDL作用下,骨髓细胞干细胞分化的平滑肌细胞能大量蓄积胆固醇酯,形成泡沫细胞,这一机制可能与细胞清道夫受体SR-A表达上调以及胆固醇逆转运蛋白ABCA1和小凹蛋白Caveolin-1表达下调相关。Objective To establish a model of smooth muscle cells differentiated from bone mesenchymal stem cells（BMSC-SMCs） in vitro and explore the relationship between scavenger receptors A（SR-A） and caveolin-1.Methods BMSCs were isolated from the femoral bone of SD rats by adherent culture.After treatment of the BMSC-SMCs with 80 mg/L ox-LDL for 72 h,Western blotting was performed to detect the expression of scavenger receptor SR-A,cell cholesterol transport protein ATP-binding cassette transporter Al（ABCA1） and caveolin-1.Results BMCS-SMCs became foam cells after treatment with ox-LDL.BMSC-SMC gave rise to more foam cell formation than VSMCsdid.Western blotting showed that treatment with 80 mg/L ox-LDL for 72 h resulted in significantly increased expression of SR-A and significantly decreased expressions of ABCA1 and caveolin-1.Conclusion Treatment of BMCS-SMCs with ox-LDL results in cholesterol ester accumulation in the cells to result in foam cells,the mechanism of which involves up-regulation of scavenger receptor SR-A expression and down-regulation of the reverse cholesterol transport protein ABCA1 and caveolin-1 expression.

关 键 词：骨髓间质干细胞 血管平滑肌细胞 清道夫受体A ATP结合盒转运子A1 小凹蛋白1 

分 类 号：R543[医药卫生—心血管疾病]