生脉散对VD大鼠行为学及海马组织NOS和神经元细胞凋亡的影响  

Effect of Shengmaisan on learning and memory abilities and hippocampal nitric oxide synthase expression and neuronal apoptosis in rats with vascular dementia

作　　者：吴莹[1] 温优良[2] 杜丽[1] 

机构地区：[1]汕头大学医学院第二附属医院,广东汕头515041 [2]广东省第二人民医院康复理疗科,广东广州510317

出　　处：《南方医科大学学报》2010年第6期1327-1329,1332,共4页Journal of Southern Medical University

基　　金：汕头市科技计划项目(2007-167)

摘　　要：目的探讨中药方剂生脉散改善VD模型大鼠认知障碍的作用机制。方法取健康Wister大鼠,随机分为正常对照组、假手术组、空白模型组、生脉散高剂量组、生脉散低剂量组和尼莫地平组。除正常对照组和假手术组外,其余各组采用双侧颈总动脉永久性结扎法,造成慢性脑灌注不足所致VD模型。术后生脉散低剂量组和高剂量组各自给予生脉散生药10 g/kg/d、30 g/kg/d,尼莫地平组予尼莫地平20 mg/kg/d灌胃,正常对照组、假手术组与空白模型组以等量生理盐水灌胃。通过Morris水迷宫观察各组大鼠的学习记忆改善情况并检测各组大鼠海马组织中NOS阳性细胞数的变化及海马神经元细胞的凋亡率。结果大鼠学习记忆能力、海马组织中NOS阳性细胞数及神经元细胞的凋亡率,生脉散低剂量组及尼莫地平组与空白模型组比较,差异有显著性意义(P<0.01);生脉散高剂量组和尼莫地平组相比,差异无显著性意义(P>0.05)。结论生脉散能明显改善VD大鼠的学习记忆能力。对缺血引起的VD具有益智作用,其作用机制可能与降低大鼠海马组织中的NOS阳性细胞数和抑制神经元凋亡有关;生脉散对血管性痴呆大鼠有一定的预防及逆转作用,但不能使其恢复至正常。Objective To observe the effect of Shengmaisan on the learning and memory abilities and the expression of nitric oxide synthase and neuron apoptosis in the hippocampus of rats with vascular dementia（VD）,and explore the mechanism of Shengmaisan for treatment of VD.Methods Wistar rats were divided randomly in to normal control group,sham-operated group,VD model group,high-dose Shengmaisan group,low-dose Shengmaisan group,and nimodipine group.In the latter 4 groups,bilateral common carotid artery occlusion（BCCAO） was performed to establish rat models of VD followed by intragastric administration of Shengmaisan at 10 or 30 g.kg-1.day-1 or nimodipine at 20 g.kg-1.day-1 accordingly.The rats in the control,sham-operated and model groups were given saline in the same manner.The improvement of learning and memory abilities of the rats was assessed using Morris water maze test,and NOS activity and neuronal apoptosis in the hippocampus were determined after the treatment.Results The learning and memory ability and hippocampal NOS activity and neuronal apoptosis in low-dose Shengmaisan group and nimodipine group were significantly different from those in the VD model group（P0.01）,but no significant differences were found between high-dose Shengmaisan group and nimodipine group（P0.05）.Conclusions Shengmaisan can significantly improve the learning and memory abilities of VD rats but may not be able to totally reverse the damage.The therapeutic effect of Shengmaisan might be related to its effect in decreasing NOS activity and inhibiting neuronal apoptosis in the hippocampus.

关 键 词：生脉散 血管性痴呆 行为学 一氧化氮合成酶 凋亡 

分 类 号：R285.5[医药卫生—中药学]