广州市某定点医院2008年5～12月手足口病收治状况及病原学研究  

An analysis of the characteristics and etiology of hand, foot and mouth disease in a Guangzhou sentinel hospital from May to December, 2008

作　　者：高璐璐[1,2] 陈清[1] 钟小祝[3] 谭细兰[3] 周杰[1] 郭江[1] 俞守义[1] 

机构地区：[1]南方医科大学公共卫生与热带医学学院流行病学系,广东广州510515 [2]广州军区空军后勤部,广东广州510620 [3]南方医科大学珠江医院感染管理科,广东广州510280

出　　处：《南方医科大学学报》2010年第6期1333-1335,共3页Journal of Southern Medical University

基　　金：卫生部“十一五”科技重大专项(20092X10001-018)

摘　　要：目的对广州市某手足口病定点收治医院2008年收治情况和病原进行分析。方法采集广州市某医院2008年5~12月手足口住院患儿的标本,接种于Vero细胞进行病毒分离,采用RT-PCR方法进行核酸鉴定,对VP1基因克隆和序列测定,并利用MEGA 4软件对VP1基因进行遗传性分析。结果共收治住院患儿309名,全年的发病主要集中在5~7月份,发病年龄集中在2~4岁,15例患儿出现并发症。检测临床标本207份,EV71阳性率为64.7%（134/207）,CoxA16阳性率14.5%（30/207）,10株广州市EV71分离株VP1基因遗传性分析全部属于C4a基因亚型。结论 EV71是2008年广州地区手足口病的最主要病原体,广州地区的EV71与目前国内多个省份及相邻国家和地区的C4a亚型的毒株一起进化和传播的。Objective To analyze the characteristics and etiology of hand,foot and mouth disease（HFMD） in a sentinel hospital of Guangzhou.Methods The epidemiological data and clinical specimens were collected from May to December,2008 for virological investigations（viral isolation,RT-PCR and molecular identification） and phylogenetic analysis.Results A total of 309 clinical cases were reported,and the incidence was the highest in 2-4-year-old children,among whom only 15 developed complications,with human enterovirus 71（HEV71） as the main pathogen（64.7%）.Phylogenetic analysis indicated that ten Guangzhou EV71 isolates belonged to Cluster C4a.Conclusions HFMD is an important infectious disease in children resulting from infections by HEV71 as the main pathogen in 2008,and the Guangzhou C4a strains co-evolved with the isolates from other provinces in China and the neighboring countries.

关 键 词：手足口病 收治状况 病原学 

分 类 号：R725.1[医药卫生—儿科]