C5-siRNA沉默补体C5对大鼠心肌缺血损伤影响的病理学研究  

Effect of C5-siRNA silencing receptor C5 on myocardial ischemia injury in rats

作　　者：成玲俐[1] 李志樑[1] 付强[1] 吴素华[2] 唐凯[2] 

机构地区：[1]南方医科大学珠江医院心内科,广东广州510282 [2]中山大学附属第一医院心内科,广东广州510080

出　　处：《南方医科大学学报》2010年第6期1486-1488,共3页Journal of Southern Medical University

基　　金：广东省自然科学基金(06021338);佛山科技局基金(200808109)

摘　　要：目的探讨小分子干扰RNA片段(siRNA)沉默补体C5表达对大鼠的缺血心肌组织病理学的影响。方法 30只SD大鼠随机分为假手术组(Sham组),心肌缺血组(Ischemia组)和C5-siRNA干预组。假手术组及心肌缺血组的大鼠冠状动脉左前降支支配区注入生理盐水(100μl/kg),C5-siRNA干预组大鼠冠脉左前降支支配区注入C5-siRNA(100μl/kg),实验结束后测定鼠的心脏指数和左心室重量指数,光镜下观察鼠心肌形态学改变及C5的免疫组织化学染色结果。结果缺血4 h后,检测3组之间的心脏指数和左心室重量指数无统计学意义的差别(P>0.05)。光镜下见干预组鼠心肌组织水肿、变性较心肌缺血组轻,心肌间质可出现少量红细胞。C5的免疫组织化学结果显示,Ischemia组与干预组的C5表达量均明显高于Sham组(P<0.001),而干预组的C5表达量虽与Ischemia组相比有所降低,但二者没有统计学意义的差别(P=0.132)。结论 C5-siRNA减轻心肌缺血的病理学损伤及降低心肌C5表达。Objective To study the effect of C5-siRNA on pathological changes after myocardial ischemia in rats.Methods Thirty healthy Sprague-Dawley rats were randomly divided into sham-operated group,ischemia group and C5-siRNA group.The cardiac ischemia models were established in ischemia group and C5-siRNA group by ligating the proximal end of the left anterior descending（LAD） coronary artery.The rats were infused with 100 μl/kg of C5-siRNA into myocardial tissue in C5-siRNA group and equal amount of normal saline in ischemia group and sham-operated group after ligating the LAD coronary artery for 30 min and then performed of ischemia for 4 hours.The cardiac index and left ventricular mass index were determined,morphological changes of myocardial tissue observed under optical microscope and the expression of C5 was detected by immunohistochemical staining and image analysis system.Results There were no statistically significant difference between the three groups in the left ventricular mass index and cardiac index in the rats after ischemia for 4 hours.Light microscopy indicated edema and degeneration of the myocardial tissue were milder in C5-siRNA group than in ischemia group,a small amount of red blood cells existed in the myocardial stroma of the former.The expression of C5 was increased more significantly in ischemia group and C5-siRNA group than in sham-operated group（P0.001）,but was decreased in C5-siRNA group more than in ischemia group with no statistically significant difference between the two groups（P=0.132）.Conclusion C5-siRNA could attenuate myocardial ischemia injury in rats by reducing inflammatory cell infiltration and expression of C5.

关 键 词：siRNA沉默补体C5 大鼠 心肌缺血/病理学 再灌注损伤/病理学 

分 类 号：R541[医药卫生—心血管疾病]