植物种传病害检测技术的研究进展  

Progress of Detecting Plant Seed-borne Diseases

作　　者：阚春月[1] 王守法[2] 杨翠云[3] 

机构地区：[1]上海海洋大学,上海201300 [2]华东理工大学,上海200237 [3]上海出入境检验检疫局,上海200135

出　　处：《安徽农业科学》2010年第15期7956-7959,共4页Journal of Anhui Agricultural Sciences

摘　　要：介绍植物种传病害的定义、分类及其检测技术的进展,着重介绍用于检测真菌性种传病害的洗涤镜检、保湿培养、致病性测定和PCR等方法以及用于检测植物病毒性病害的常见方法,如症状观察(包括鉴别寄主反应)、血清学反应、分子生物学方法等。最后,对种传病害的检测方法作了总结和展望。The definition and classification of plant seed-borne diseases were introduced.And the progress of detecting plant seed-borne diseases was discussed.The techniques used in detecting the fungal diseases such as washing microscopic examination,moisture culture,inoculation,PCR method were highlighted.Meanwhile,the techniques used in detecting the viral diseases such as symptom observation（including differential host test）,serological methods,molecular biology methods were also described in detail.And at the end of the paper,the tendency of development in detecting plant seed-borne diseases and its prospect were given.

关 键 词：种传病害 检测技术 洗涤镜检 保湿培养 血清学方法 分子生物学方法 

分 类 号：S432[农业科学—植物病理学]