黄褐毛忍冬总皂苷对卵清蛋白致敏小鼠肠道炎症因子和抗炎因子的影响  

Effects of fulvotomentoside on inflammatory factors and antiinflammatory factors in intestine of ovalbumin-sensitized BALB/c mice

作　　者：白枫[1] 黎海芪[1] 

机构地区：[1]重庆医科大学附属儿童医院儿童保健科,400014

出　　处：《中华儿科杂志》2010年第7期520-525,共6页Chinese Journal of Pediatrics

基　　金：《中华儿科杂志》第二届百利儿科科研基金资助项目

摘　　要：目的 观察黄褐毛忍冬总皂苷（Ful）对卵清蛋白（OVA）致敏小鼠肠道炎症因子和抗炎因子的影响.方法 24只雌性BALB/c小鼠随机取16只,采用卵清蛋白致敏和激发构建食物过敏模型,均分为2组,即食物过敏组（FA组）和Ful干预组（Ful组）.Ful组小鼠自造模第20天起每日皮下注射Ful 200 mg/kg,共22 d.另8只小鼠作为正常对照组（NS组）.采用逆转录-聚合酶链反应（RT-PCR）法检测小鼠空肠组织中转化生长因子β1（TGF-β1）、白细胞介素6（IL-6）、白细胞介素17A（IL-17A）、叉头蛋白3-T细胞转录因子（Foxp3）mRNA表达;免疫组织化学法检测小鼠空肠组织中TGF-β1、IL-6、IL-17A蛋白表达;检测空肠中髓过氧化物酶（MPO）活性代表中性粒细胞活化水平.结果 FA组小鼠空肠组织中TGF-β1、IL-6、IL-17A的mRNA[（0.370±0.013）、（0.475±0.015）]和TGF-β1、IL-6、IL-17A蛋白表达水平[（53 075.70±20 727.06）、（256 881.66±36 561.79）、（435 064.25±69 911.48）]均增高,Foxp3 mRNA（0.231±0.014）.经黄褐毛忍冬总皂苷干预后,小鼠空肠组织TGF-β1表达未下降,但IL-6、IL-17A的mRNA[（0.196±0.005）、（0.204±0.008）]和蛋白表达水平[（114 040.30±20 295.25）、（218 200.74±30 077.69）]均明显降低,而Foxp3 mRNA（0.578±0.021）表达明显增高.空肠组织中MPO水平各组间比较差异无统计学意义（P＞0.05）..结论 食物过敏发生时肠道内存在以IL-6、IL-17A表达增高为主的炎症反应.Ful可有效降低OVA致敏小鼠肠道炎症因子IL-6、IL-17A的过度表达,显著增强调节性T细胞特异性转录因子Foxp3的表达,从改善OVA诱导的肠道炎症.Objective To investigate the effects of fulvotomentoside （Ful） on inflammatory factors and antiinflammatory factors in intestine of ovalbumin （OVA）-sensitized BALB/c mice, and to explore the mechanisms of its anti-food allergy effect. Method Twenty-four female BALB/c mice aged 6 weeks fed with ovalbumin-free feed were randomly divided into 3 groups, food allergy （FA） group, Ful group and normal saline （NS） group. Mice in FA and Ful groups were sensitized intraperitoneally two times with OVA and challenged intragastrically with OVA. Mice in Ful group were treated with 200 mg/kg of Ful by subcutaneous injection once daily for 22 days. The mice in FA and NS groups were used as positive control and negative control, respectively, and were treated with normal saline solution by subcutaneous injection for 22 days. Just 48 hours after the last challenge, the mice in each group were sacrificed and specimens of jejunum were taken. The mRNA expressions of transforming growth factor β1 （ TGF-β1 ）, interleukin-6 （IL-6）, interleukin-17A （IL-17A） and forkhead box P3 （Foxp3） in jejunum were detected by reverse transcription-PCR （RT-PCR）. The protein expressions of TGF-β1, IL-6, and IL-17A in jejunum were detected by immunohistochemical method. The activation of neutrophils in jejunum was assayed by the levels of MPO. Result The expressions of TGF-β1, IL-6, IL-17A mRNA [ （0. 370 ± 0. 013 ）, （0. 475 ±0. 015）, （0. 541 ±0. 013） ]and related protein [ （53 075.70 ±20 727.06）, （256 881.66 ±36 561.79）,（435 064.25 ±69 911.48）] in jejunum were increased and the Foxp3 mRNA [（0.231 ±0.014） vs.（0. 365 ±0. 015） ] expression was decreased in group FA. After the treatment with Ful, IL-6 and IL-17A mRNA [ （0. 196 ±0. 005）, （0. 204 ±0. 008） ] and protein [ （ 114 040. 30 ±20 295.25）, （218 200. 74 ±30 077.69） ] expressions were decreased and Foxp3 mRNA （0. 578 ±0. 021 ） expression was increased,and no change of TGF-β1 expression was unchanged.

关 键 词：中草药 食物过敏 肠黏膜 小鼠 炎症因子 抗炎因子 黄褐毛忍冬总皂苷 

分 类 号：R285.5[医药卫生—中药学]