蔗糖及天然有机物对银杏愈伤组织合成黄酮的影响  

Effect of Sucrose and Natural Organic Affixtures on Flavonoids Biosynthesis of Ginkgo Callus

作　　者：胡燕梅[1] 郭云贵[1] 蒋细旺[2] 余群娣[1] 崔鹏[1] 

机构地区：[1]武汉生物工程学院生物工程系,湖北武汉430415 [2]江汉大学生命科学学院,湖北武汉430056

出　　处：《西南农业学报》2010年第3期690-693,共4页Southwest China Journal of Agricultural Sciences

基　　金：湖北省教育厅科学技术研究资助项目(B20094008);武汉市高校科学研究资助项目(2008K079)

摘　　要：研究了蔗糖和其它5种有机附加物对银杏愈伤组织生长及黄酮积累的影响。结果表明:供试浓度30 g/L蔗糖最有利于愈伤组织生长,60 g/L蔗糖加入培养基中黄酮含量最高;10 mL/L椰乳、1 mL/L芦荟汁、15 mL/L蜂蜜、50 mL/L银杏胚乳汁能促进愈伤组织生物量的提高,而银杏叶汁对愈伤组织生长作用不明显;200 mL/L椰乳、5 mL/L芦荟汁、1 mL/L蜂蜜、32.6 mL/L银杏叶汁能有效提高黄酮含量,但银杏胚乳汁的作用不明显。The effects of sucrose and other five natural organic affixtures on callus growth and flavonoids content were reported.Results showed： the test of 30 g/L sucrose was the best for callus growth and the test of 60 g/L sucrose was the highest for flavonoids content respectively.The callus growth index was significantly improved by other five natural organic affixtues including 10 mL/L coconut milk,1 mL/L asparagus juice.15 mL/L honey and 50 mL/L ginkgo endosperm juice,but not significantly by ginkgo leaf juice.The flavonoids content was significantly improved by 200 mL/L coconut milk,5 mL/L asparagus juice,1 mL/L honey,32.6 mL/L ginkgo leaf juice,but not significantly by ginkgo endosperm juice.

关 键 词：银杏(Ginkgo biloba L.) 愈伤组织 黄酮 蔗糖 蜂蜜 椰子乳(CM) 芦荟汁 

分 类 号：Q949.640.5[生物学—植物学] Q943.1