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机构地区:[1]海南省农业科学院热带果树研究所,海南海口571100 [2]海南大学环境与植物保护学院,海南儋州571737
出 处:《西南农业学报》2010年第3期752-755,共4页Southwest China Journal of Agricultural Sciences
基 金:国家科技支撑项目"热带果树重大病虫害防控技术研究与示范"(2007BAD48B05);国家科技基础条件平台"热带作物种质资源标准化整理;整合及共享试点"(2005DKA21000-5-32)
摘 要:对番木瓜Carica papaya L.炭疽病果组织进行分离、病原菌鉴定及其生物学特性测定。结果表明,该病原菌初步鉴定为胶孢炭疽Colletotrichum gloeosporioides Penz.,在PDA培养基上生长速度最快。菌丝生长和分生孢子萌发的适宜温度分别为20~30℃和25~30℃,适宜pH值分别为7~9和6~10。供试的9种碳源中以葡萄糖和蔗糖有利于病原菌的生长,以蔗糖有利于该菌分生孢子的萌发;供试的9种氮源中,以蛋白胨和酵母浸膏最有利于菌丝生长,以酵母浸膏最有利于该菌分生孢子的萌发。完全黑暗与光暗交替有利于菌丝生长,光暗交替有利于孢子萌发。The identification and biological characterization of the pathogen isolated from papaya(Carica papaya L.)fruit induced by anthracnose were studied in this paper.The results showed that the pathogen was Colletotrichum gloeosporioides Penz.,growing most quickly on PDA under experimental conditions.The optimum temperature for its mycelial growth and conidial germination were 20-30 ℃and 25-30 ℃ separately,and the optimum pH were 7-9 and 6-10.The glucose,sucrose were more beneficial for the mycelial growth and the latter more beneficial for conidial germination than the other carbon sources.To the nitrogen sources,the peptone and yeast extract was more favorable for mycelial growth than the others and the yeast extract was the best for its conidial germination.Besides,the condition of constant darkness and light-dark alternation were effective for mycelial growth,but just the light-dark alternation was conducive for the conidial germination.
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