应用形态学与RAPD标记对朝天椒遗传多样性的分析  

Morphology Comparison and RAPD Genetic Diversity Analysis of Seven Varieties of Capsicum frutescen

作　　者：蒋向辉[1,2,3] 佘朝文[1,2,3] 许栋[1] 张青桦[1] 赵旺[1] 

机构地区：[1]怀化学院生命科学系 [2]怀化学院民族药用植物资源研究与利用湖南省重点实验室 [3]怀化学院湘西药用植物与民族植物学湖南省高校重点实验室,湖南怀化418008

出　　处：《西南农业学报》2010年第3期810-813,共4页Southwest China Journal of Agricultural Sciences

基　　金：湖南省科研计划重点资助项目(2009FJ2008);湖南省教育厅资助项目(09C755);民族药用植物资源研究与利用湖南省重点实验室项目(SYSXM200909)

摘　　要：为阐明朝天椒种质资源的遗传基础,结合运用形态标记与RAPD标记对7份朝天椒种质资源的遗传多样性进行分析。结果表明,形态标记以10.25的遗传距离将7个朝天椒辣椒种质材料分成了5类,RAPD分子标记以15的遗传距离将供试辣椒种质分成4类,扩增片段的多态性比例为24.4%,扩增多态性位点多,材料间遗传相似系数变幅不明显,对于亲缘关系较近的品种,从分子水平和传统形态学方法进行聚类分析的结果相似。To clarify the genetic basis of Capsicum germplasm resources,we commonly refered to quantitative character genetic mechanisms,however,it was easily to be affected by environmental condition and showed low heritability.It＇s hard to identify the markers of homology,and the observation standard was difficult to grasp,and with a heavy workload.The morphology marker with DNA molecular marker was combined to investigate the genetic diversity of Capsicum germplasm resources.On the basis of morphology comparison,RAPD molecular marker technique was used to analyse the genetic diversity of seven capsicum varieties from different eco-environment.The result showed the same conclusion we got on DNA molecular level as the traditional morphology research.

关 键 词：朝天椒 形态学标记 RAPD标记 遗传多样性 聚类分析 

分 类 号：S641.3[农业科学—蔬菜学]