纹瓣兰无菌播种快繁技术研究  

Study on Technology of Aseptic Sowing and Rapid Propagation of Cymbidium aloifolium

作　　者：张铁[1,2] 李洪超[1,2] 王国芬 

机构地区：[1]文山学院生化系,云南文山663000 [2]文山州生物资源开发研究中心,云南文山663000 [3]文山民族职业技术学校,云南文山663000

出　　处：《西南农业学报》2010年第3期993-995,共3页Southwest China Journal of Agricultural Sciences

摘　　要：以纹瓣兰[Cymbidium aloifolium(L.)Sw]的种子为外植体,采用种子→原球茎→完整植株的途径进行繁殖。结果表明:种子在MS+6-BA0.2 mg/L+AC 1.0 g/L+蔗糖30 g/L培养基培养30 d左右,可形成原球茎;原球茎在1/2MS+6-BA4 mg/L+KT0.5 mg/L+蔗糖30 g/L的培养基上增殖效果最好;原球茎接种于1/2MS+6-BA0.1 mg/L+NAA0.5 mg/L+KT0.5 mg/L+蔗糖30 g/L培养基上培养4周后,再转入1/2MS+6-BA0.5 mg/L+NAA0.1 mg/L+AC2.0 g/L+香蕉泥80 g/L的培养基上培养30d,可诱导形成完整的植株。Seeds of Cymbidium aloifolium（L.） Sw could be used as explants to propagate by the way of ＇seed →protocorm →the whole plant＇.The results showed that,when cultured for about 30 days in the medium ＇MS ＋ 6-BA 0.2 mg/L ＋ AC 1.0 g/L ＋ sucrose 30 g/L＇,seeds could develop into protocorms,and propagate best in ＇1/2MS ＋ 6-BA 4 mg/L ＋KT 0.5 mg/L ＋ sucrose 30 g/L＇;protocorms were subcultured in ＇1/2MS ＋ 6-BA 0.1 mg/L ＋ NAA 0.5 mg/L ＋ KT 0.5 mg/L ＋ sucrose 30 g/L＇ for 4 weeks and transfered into ＇1/2MS ＋ 6-BA 0.5 mg/L ＋ NAA 0.1 mg/L ＋ AC 2.0 g/L ＋ mashed banana 80 g/L＇,after 30 days,young plantlets were induced and were able to develop into whole plants.

关 键 词：纹瓣兰 无菌播种 原球茎 

分 类 号：S682.31[农业科学—观赏园艺]