腹毛目纤毛虫大尾柱虫腹皮层纤毛器基部微管的荧光标记  

Ciliature Base-Associated Microtubules in the Ventral Cortex of Urostyla grandis(Ciliaphora,Hypotrichida) Revealed by Fluorescent Labeling

作　　者：刘兰侠[1] 余齐耀[1] 俞丽丽[1] 栾菊敏[1] 顾福康[1] 

机构地区：[1]华东师范大学生命科学学院,上海200062

出　　处：《四川动物》2010年第4期576-578,F0002,共4页Sichuan Journal of Zoology

基　　金：国家自然科学基金资助项目(30770238)

摘　　要：应用荧光紫杉醇直接荧光标记,显示腹毛目纤毛虫大尾柱虫Urostyla grandis腹皮层纤毛器微管胞器由口围带、波动膜、额腹横棘毛和左、右缘棘毛等纤毛器微管、纤毛器基部附属微管等组成。其中,口围带小膜托架及其相联系的肋壁微管和波动膜基体托架,额棘毛基部前纵微管束、后纵微管束及横棘毛基部前纵微管束,中腹棘毛及左、右缘棘毛基部前纵微管束、后纵微管束和横微管束,是该纤毛虫皮层纤毛器基部的主要附属微管。据结果推测,尽管腹毛目纤毛虫的纤毛器基部微管具有相同的结构成分,但其结构的组成、分化特征、定位和定向、发达程度等均有差异。所得结果为进一步说明纤毛虫细胞皮层纤毛器的形态及其微管建构的多样性提供了新的证据资料。The ciliature microtubular organelles in the ventral cortex of Urostyla grandis were visualized by direct fluorescent labeling of FLUTAX. The organelles consist of the adoral zone of membranelles （ AZM）, undulating membranes （UM）, frontal-midventral-transverse cirri （FVTC）, left and right marginal cirri （L-and RMC ）, and the base-associated microtubules. These base-associated microtubules are comprised of membranelle brackets of AZM, its associated oral ribwall microtubules, base body brackets of UM, anterior longitudinal microtubules （ALM） and posterior longitudinal microtubules （PLM） of frontal cirri （ FC）, ALM of TC, ALM, PLM and transverse microtubules （TM） of midventral cirri （VC） and L-and RMC. According to the results, despite the fact that ciliature base-associated microtubules of hypotrichous ciliates have a basic structural component, the composition, differentiation characteristics, location and orientation, and level of development are different. Therefore, the results provide new evidence for the morphology of cortex ciliated cells and diversity of microtubules construction.

关 键 词：腹毛目纤毛虫 大尾柱虫 纤毛器基部微管 荧光标记 

分 类 号：Q954.4[生物学—动物学]