高效液相色谱法测定白术挥发油中苍术酮β-桉叶醇榄香烯的含量  

HPLC determination of atractylon, β-eudesmol and β-elemene in volatile oil of Atractylodes macrocephala Koidz

作　　者：阎克里[1] 朱秀卿[2] 赵丽[1] 

机构地区：[1]山西省肿瘤医院药物检测室,太原030013 [2]山西省肿瘤医院中药制剂室,太原030013

出　　处：《中国药物与临床》2010年第7期751-753,共3页Chinese Remedies & Clinics

基　　金：山西省卫生厅科技攻关项目(200833)

摘　　要：目的建立高效液相色谱法测定白术挥发油中苍术酮、β-桉叶醇、β-榄香烯含量分析方法。方法采用HypersilODS2（4.6mm×250mm,5μm）色谱柱;流动相为乙腈-水（A）-水（B）60∶40,线性梯度洗脱（0~30min,40%B→10%B;30~40min,10%B→10%B）;流速为1.0ml/min,柱温室温,检测波长200nm。结果苍术酮浓度在0.10~1.00mg/ml,β-桉叶醇浓度在2.00~600.00μg/ml,β-榄香烯浓度在0.70~42.00μg/ml范围内呈良好线性关系（r≥0.9998）;3个组分低、中、高浓度的平均加样回收率在99.7%~101.2%,RSD（n=3）均〈2.3%;重复性良好,RSD（n=6）均〈1.6%。结论本方法简便、准确可靠,可用于白术挥发油中苍术酮、β-桉叶醇、β-榄香烯的含量测定。Objective To develop a technique for determination of atractylon, β-eudesmol and β-elemene in Atractylodes macrocephala Koidz volatile oil using high performance liquid chromatography （HPLC）. Methods A Hypersil ODS-2 column （250 mm×4.6 nm, 5 μm） was used. The mobile phase consisted of acetonitrile-water （A） -wa- ter （B） （60：40）, with linear gradient elution （0-30min, 40%B→10%B; 30-40min, 10%B→10%B） at a flow rate of 1.0 ml/min. The column was kept at room temperature and UV detection wavelength was set to 200nm. Results The con- centrations of Atractylon （0.1~1.00 mg/ml）, β-eudesmol （2.00~600.00 μg/ml） and β-elemene （0.70~42.00 μg/ml） showed good linearity （r≥0.9998）. The mean sample recovery of the three groups at low, medium and high concentra- tion levels was between 99.7% and 101.2%, with all of their RSD values below 2.3% （n=3）. The HPLC determination was well reproducible with all RSD values smaller than 1.6% （n=6）. Conclusion The HPLC method appeared simple, accurate and reliable, and may therefore warrant its use for determination of atractylon, β-eudesmol and β-elemene in Atractylodes macrocephala Koidz volatile oil.

关 键 词：色谱 高效液相法 含量测定 油类 挥发 

分 类 号：R284.1[医药卫生—中药学]